| see this table in Ki's | see this table in - D G (kcal/mol) | see this table in - D G (kj/mol) |
Equilibrium Constants for 25 X18 OMTKY3 P1 Variants; pH 8.3, t=21 +2 oC
|
|
CHYM |
PPE |
CARL |
SGPA |
SGPB |
HLE |
|
Gly |
6.6 x 10 6 |
9.0 x 10 8 |
6.4 x 10 8 |
4.5 x 10 7 |
1.2 x 10 7 |
2.2 x 10 7 |
|
Ala |
6.1 x 10 7 |
4.2 x 10 10 |
2.0 x 10 10 |
1.9 x 10 9 |
3.6 x 10 8 |
1.0 x 10 9 |
|
Abu |
1.1 x 10 9 |
3.3 x 10 11 |
1.0 x 10 11 |
1.0 x 10 10 |
2.3 x 10 9 |
1.4 x 10 10 |
|
Ser |
4.2 x 10 7 |
8.6 x 10 8 |
2.4 x 10 9 |
1.6 x 10 8 |
5.0 x 10 7 |
3.3 x 10 7 |
|
Cys |
2.4 x 10 9 |
2.5 x 10 10 |
3.3 x 10 11 |
3.3 x 10 11 |
5.7 x 10 10 |
7.1 x 10 9 |
|
Ape |
2.2 x 10 10 |
2.4 x 10 11 |
1.4 x 10 11 |
8.3 x10 10 |
1.2 x 10 10 |
1.0 x 10 10 |
|
Val |
1.4 x 10 8 |
8.8 x 10 9 |
8.9 x 10 8 |
2.1 x 10 9 |
3.3 x 10 8 |
1.4 x 10 9 |
|
Hse |
2.5 x 10 9 |
7.5 x 10 9 |
5.6 x 10 10 |
6.5 x 10 9 |
1.7 x 10 9 |
2.6 x 10 8 |
|
Thr |
9.8 x 10 7 |
2.9 x 10 10 |
2.6 x 10 10 |
2.0 x 10 9 |
2.5 x 10 8 |
1.3 x 10 9 |
|
Pro |
6.7 x 10 4 |
5.9 x 10 5 |
7.9 x 10 4 |
4.6 x 10 4 |
3.6 x 10 4 |
1.9 x 10 5 |
|
Ahx |
8.0 x 10 10 |
1.9 x 10 11 |
6.7 x 10 10 |
2.3 x 10 11 |
2.1 x 10 10 |
9.9 x 10 8 |
|
Leu |
1.9 x 10 11 |
4.2 x 10 10 |
3.4 x 10 10 |
3.0 x 10 11 |
5.6 x 10 10 |
6.1 x 10 9 |
|
Ile |
9.9 x 10 7 |
5.9 x 10 9 |
1.4 x 10 8 |
1.5 x 10 8 |
2.9 x 10 7 |
2.1 x 10 10 |
|
Met |
1.0 x 10 11 |
1.3 x 10 10 |
8.1 x 10 10 |
2.6 x 10 11 |
2.7 x 10 10 |
7.3 x 10 8 |
|
Asn |
6.6 x 10 8 |
6.9 x 10 7 |
2.7 x 10 9 |
2.6 x 10 8 |
1.8 x 10 8 |
9.0 x 10 5 |
|
Asp |
1.0 x10 6 |
7.3 x 10 4 |
1.6 x 10 7 |
5.1 x 10 6 |
3.9 x 10 6 |
1.6 x 10 4 |
|
Ahp |
3.3 x 10 11 |
2.4 x 10 8 |
8.9 x 10 10 |
4.7 x 10 11 |
2.8 x 10 10 |
1.3 x 10 8 |
|
Lys |
1.0 x 10 8 |
4.6 x 10 4 |
1.2 x10 8 |
4.0 x 10 8 |
2.6 x 10 8 |
3.8 x 10 5 |
|
Gln |
1.3 x 10 9 |
4.3 x 10 7 |
1.3 x 10 10 |
1.2 x 10 9 |
6.9 x 10 8 |
2.3 x 10 7 |
|
Glu |
2.5 x 10 6 |
8.4 x 10 4 |
8.6 x 10 8 |
7.9 x 10 6 |
2.3 x 10 6 |
4.1 x 10 4 |
|
His |
1.2 x 10 9 |
3.7 x 10 4 |
1.3 x 10 10 |
2.6 x 10 9 |
3.1 x 10 9 |
1.1 x 10 5 |
|
Phe |
2.4 x 10 12 |
4.9 x 10 4 |
1.2 x 10 10 |
1.8 x 10 11 |
5.5 x 10 9 |
9.9 x 10 5 |
|
Arg |
2.2 x 10 8 |
4.8 x 10 3 |
2.4 x 10 8 |
8.2 x 10 7 |
1.9 x 10 8 |
3.3 x 10 4 |
|
Tyr |
8.1 x 10 12 |
7.5 x 10 3 |
1.6 x 10 10 |
1.0 x 10 11 |
3.3 x 10 9 |
8.3 x 10 4 |
|
Trp |
3.3 x 10 12 |
2.5 x 10 4 |
2.6 x 10 9 |
8.9 x 10 9 |
2.4 x 10 9 |
1.8 x 10 4 |
return to Laskowski Lab website
| see this table in Ka's | see this table in - D G (kcal/mol) | see this table in - D G (kj/mol) |
Equilibrium Constants for 25 X18 OMTKY3 P1 Variants; pH 8.3, t=21 +2 oC
|
|
CHYM |
PPE |
CARL |
SGPA |
SGPB |
HLE |
|
Gly |
1.5 x 10 –7 |
1.1 x 10 –9 |
1.6 x 10 –9 |
2.2 x 10 –8 |
8.3 x 10 –8 |
4.5 x 10 –8 |
|
Ala |
1.6 x 10 –8 |
2.4 x 10 –11 |
5.0 x 10 –11 |
5.3 x 10 –10 |
2.8 x 10 –9 |
1.0 x 10 –9 |
|
Abu |
9.1 x 10 –10 |
3.0 x 10 -12 |
1.0 x 10 –11 |
1.0 x 10 –10 |
4.3 x 10 –10 |
7.1 x 10 –11 |
|
Ser |
2.4 x 10 –8 |
1.2 x 10 –9 |
4.2 x 10 –10 |
6.3 x 10 –9 |
2.0 x 10 –8 |
3.0 x 10 –8 |
|
Cys |
4.2 x 10 –10 |
4.0 x 10 –11 |
3.0 x 10 –12 |
3.0 x 10 –12 |
1.8 x 10 –11 |
1.4 x 10 –10 |
|
Ape |
4.5 x 10 –11 |
4.2 x 10 –12 |
7.1 x 10 –12 |
1.2 x 10 –11 |
8.3 x 10 –11 |
1.0 x 10 –10 |
|
Val |
7.1 x 10 –9 |
1.1 x 10 –10 |
1.1 x 10 –9 |
4.8 x 1 –10 |
3.0 x 10 –9 |
7.1 x 10 –11 |
|
Hse |
4.0 x 10 –10 |
1.3 x 10 –10 |
1.8 x 10 –11 |
1.5 x 10 –10 |
5.9 x 10 –10 |
3.8 x 10 –9 |
|
Thr |
1.0 x 10 –8 |
3.4 x 10 –11 |
3.8 x 10 –11 |
5.0 x 10 –10 |
4.0 x 10 –9 |
7.7 x 10 –10 |
|
Pro |
1.5 x 10 –5 |
1.7 x 10 –6 |
1.3 x 10 –5 |
2.2 x 10 -5 |
2.8 x 10 –5 |
5.3 x 10 –6 |
|
Ahx |
1.3 x 10 –11 |
5.3 x 10 –12 |
1.5 x 10 –11 |
4.3 x 10 –12 |
4.8 x 10 –11 |
1.0 x 10 –9 |
|
Leu |
5.3 x 10 –12 |
2.4 x 10 –11 |
2.9 x 10 –11 |
3.3 x 10 –12 |
1.8 x 10 –11 |
1.6 x 10 –10 |
|
Ile |
1.0 x 10 –8 |
1.7 x 10 –10 |
7.1 x 10 –9 |
6.7 x 10 –9 |
3.4 x 10 –8 |
4.8 x 10 –11 |
|
Met |
1.0 x 10 –11 |
7.7 x 10 –11 |
1.2 x 10 –11 |
3.8 x 10 –12 |
3.7 x 10 –11 |
1.4 x 10 –9 |
|
Asn |
1.5 x 10 –9 |
1.4 x 10 –8 |
3.7 x 10 –10 |
3.8 x 10 –9 |
5.6 x 10 –9 |
1.1 x 10 –6 |
|
Asp |
1.0 x 10 –6 |
1.4 x 10 –5 |
6.3 x 10 –8 |
2.0 x 10 –7 |
2.6E x 10 –7 |
6.3 x 10 –5 |
|
Ahp |
3.0 x 10 –10 |
4.2 x 10 –9 |
1.1 x 10 –11 |
2.1 x 10 –12 |
3.6 x 10 –11 |
7.7 x 10 –9 |
|
Lys |
1.0 x 10 –8 |
2.2 x 10 –5 |
8.3 x 10 –9 |
2.5 x 10 –9 |
3.8 x 10 –9 |
2.6 x 10 –6 |
|
Gln |
7.7 x 10 –10 |
2.3 x 10 –8 |
7.7 x 10 –11 |
8.3 x 10 –10 |
1.4 x 10 –9 |
4.3 x 10 –8 |
|
Glu |
4.0 x 10 –7 |
1.2 x 10 –5 |
1.2 x 10 –9 |
1.3 x 10 –7 |
4.3 x 10 –7 |
2.4 x 10 –5 |
|
His |
8.3 x 10 –10 |
2.7 x 10 –5 |
7.7 x 10 –11 |
3.8 x 10 –10 |
3.2 x 10 –10 |
9.1 x 10 –6 |
|
Phe |
4.2 x 10 –13 |
2.0 x 10 –5 |
8.3 x 10 –11 |
5.6 x 10 –12 |
1.8 x 10 –10 |
1.0 x 10 –6 |
|
Arg |
4.5 x 10 –9 |
2.1 x 10 –4 |
4.2 x 10 –9 |
1.2 x 10 –8 |
5.3 x 10 –9 |
3.0 x 10 –5 |
|
Tyr |
1.2 x 10 –13 |
1.3 x 10 –4 |
6.3 x 10 –11 |
1.0 x 10 –11 |
3.0 x 10 –10 |
1.2 x 10 –5 |
|
Trp |
3.0 x 10 –13 |
4.0 x 10 –4 |
3.8 x 10 –10 |
1.1 x 10 –10 |
4.2 x 10 –10 |
5.6 x 10 –5 |
return to Laskowski Lab website
Table 1 from Lu et. al., J. Mol. Biol. 226: 441-461 (1997)
Equilibrium Constants for 25 X18 OMTKY3 P1 Variants; pH 8.3, t=21 +2 oC
CHYM PPE CARL SGPA SGPB HLE Gly 9.14 12.00 11.81 10.26 9.49 9.84 Ala 10.44 14.24 13.81 12.44 11.47 12.07 Abu 12.12 15.44 14.75 13.41 12.55 13.60 Ser 10.22 11.98 12.58 11.00 10.32 10.08 Cys 12.58 13.94 15.44 15.44 14.42 13.21 Ape 13.87 15.26 14.94 14.64 13.51 13.41 Val 10.92 13.33 12.00 12.50 11.42 13.60 Hse 12.60 13.24 14.41 13.16 12.37 11.28 Thr 10.71 14.03 13.96 12.47 11.26 12.22 Pro 6.47 7.74 6.57 6.25 6.11 7.08 Ahx 14.62 15.12 14.51 15.23 13.84 12.06 Leu 15.12 14.24 14.12 15.39 14.41 13.12 Ile 10.72 13.10 10.92 10.96 10.00 13.84 Met 14.75 13.56 14.62 15.30 13.98 11.88 Asn 11.82 10.51 12.64 11.28 11.07 7.98 Asp 8.04 6.52 9.66 8.99 8.84 5.64 Ahp 15.44 11.23 14.68 15.65 14.01 10.88 Lys 10.72 6.25 10.83 11.53 11.28 7.48 Gln 12.22 10.23 13.56 12.17 11.85 9.87 Glu 8.58 6.60 11.98 9.25 8.53 6.18 His 12.17 6.12 13.56 12.62 12.72 6.76 Phe 16.60 6.29 13.51 15.09 13.06 8.04 Arg 11.18 4.94 11.23 10.61 11.10 6.06 Tyr 17.31 5.19 13.68 14.75 12.76 6.59 Trp 16.78 5.90 12.62 13.34 12.58 5.70
return to Laskowski Lab website
Table 1 from Lu et. al., J. Mol. Biol. 226: 441-461 (1997)
P1 Data in (negative) Free Energies (kcal/mol)
see this table in Ka's
see this table in Ki's
see this table in - D G (kj/mol)
P1 Data in (negative) Free Energies (kJ/mol)
see this table in Ka's
see this table in Ki's
see this table in - D G (kcal/mol)
Equilibrium Constants for 25 X18 OMTKY3 P1 Variants; pH 8.3, t=21 +2 oC
|
|
CHYM |
PPE |
CARL |
SGPA |
SGPB |
HLE |
|
Gly |
38.25 |
50.23 |
49.40 |
42.93 |
39.71 |
41.18 |
|
Ala |
43.67 |
59.59 |
57.78 |
52.05 |
47.99 |
50.48 |
|
Abu |
50.71 |
64.61 |
61.70 |
56.09 |
52.51 |
56.91 |
|
Ser |
42.76 |
50.12 |
52.62 |
46.02 |
43.18 |
42.17 |
|
Cys |
52.62 |
58.32 |
64.61 |
64.61 |
60.33 |
55.26 |
|
Ape |
58.01 |
63.83 |
62.52 |
61.25 |
56.54 |
56.09 |
|
Val |
45.69 |
55.78 |
50.20 |
52.29 |
47.78 |
56.91 |
|
Hse |
52.71 |
55.39 |
60.29 |
55.04 |
51.78 |
47.20 |
|
Thr |
44.82 |
58.69 |
58.42 |
52.17 |
47.11 |
51.12 |
|
Pro |
27.07 |
32.37 |
27.47 |
26.15 |
25.56 |
29.61 |
|
Ahx |
61.16 |
63.26 |
60.73 |
63.73 |
57.90 |
50.46 |
|
Leu |
63.26 |
59.59 |
59.07 |
64.38 |
60.29 |
54.89 |
|
Ile |
44.85 |
54.81 |
45.69 |
45.86 |
41.86 |
57.90 |
|
Met |
61.70 |
56.73 |
61.19 |
64.03 |
58.51 |
49.72 |
|
Asn |
49.47 |
43.97 |
52.90 |
47.20 |
46.31 |
33.40 |
|
Asp |
33.65 |
27.28 |
40.41 |
37.62 |
36.97 |
23.58 |
|
Ahp |
64.61 |
47.01 |
61.42 |
65.47 |
58.60 |
45.51 |
|
Lys |
44.87 |
26.15 |
45.32 |
48.25 |
47.20 |
31.30 |
|
Gln |
51.12 |
42.82 |
56.73 |
50.93 |
49.58 |
41.29 |
|
Glu |
35.89 |
27.62 |
50.12 |
38.69 |
35.68 |
25.87 |
|
His |
50.93 |
25.62 |
56.73 |
52.81 |
53.24 |
28.28 |
|
Phe |
69.44 |
26.31 |
56.54 |
63.13 |
54.64 |
33.63 |
|
Arg |
46.79 |
20.65 |
47.01 |
44.39 |
46.44 |
25.35 |
|
Tyr |
72.41 |
21.74 |
57.24 |
61.70 |
53.39 |
27.59 |
|
Trp |
70.22 |
24.67 |
52.81 |
55.81 |
52.62 |
23.87 |