Biologicals

Biological Safety

Purdue Biological Safety Manual
       The section dealing with WHAT EXACTLY are biohazards is
        Appendix D: Biological Agent Risk Classification.
               It's long, and quoted pretty directly from
               NIH. It is required.
       The sections dealing with BIO-WASTE TREATMENT AND PICK-UP are
        on the page numbered 24 and in Appendix B.

List of Select Agents from CDC. Agents having potential for use in bioterrorism. You (grad students, postdocs, faculty members) can now go to prison for incorrect handling of select agents.

UPDATE 3/23/05    (Federal Register final rules on possession, use, and transfer of agents)
New Health and Human Services Final Rule
New Ag Dept Final Rule

Blood-n-Guts clean-up. (OK, it's more likely to be blood-or-puke clean up.) If you discover a biological mess in a hallway or restroom, classroom ... an academic campus building (not residence halls -- they have other arrangements), notify your building deputy (for BRWN and WTHR call the building deputy Darrel Dirksen or someone on his staff in the Chem Shop 45209 or 45211). If you cannot reach the building deputy or he/she cannot respond, or during other hours phone:

	Mon	Tues	Wed	Thu	Fri	Sat	Sun
5 am - 1:30 pm						waiting for revision
1:30 pm - 4 pm						pending hire/appointment of new
4 pm - 11 pm						Bldg Serv Director 1/18/08
10:30 pm - 7 am						mean time phone 47105 Bldg Serv info for blood clean up.

IF POSSIBLE THE PERSON who bled (or puked, or whatever) should clean it up, since they are not at risk from their own body fluids. If practical, cordon off the area until the person responsible for the body fluid(s) can return and mop it up. .

Call PUPD 48224 as a last resort and during times not covered above. They will relay the message or tell you who to call.
For large spills of human blood or material which might contain human blood, calling it an emergency is appropriate if there is not a way to restrict the area and protect students and staff from walking through it and coming into contact with bloodborne pathogens. Calling 911 is warranted for immediate attention in this case.

Agent Inventory (form, PDF, must have (free) Acrobat Reader, or telephone REM at 41496 or 47968 and request. Agent Inventory info.
Bio waste disposal
Biosafety Cabinet Installation/Moving SOP
BBP training -- Required of those working with unfixed human tissue or primary cell lines, human blood or blood products. Commercially obtained cell lines do not trigger the training requirement (at the discretion of the Biosafety Officer). But these will trigger the training requirement if they are obtained from another lab, due to the potential of disease contamination. Note: commercially obtained cell lines used in research must be registered with the PU Institutional Biosafety Committee where we require the PI to review BSL2 lab practices.
Institutional Biosafety Committee VP Research Office, licensing requirements for any and all research or teaching work with any and all biohazards.
IBC Form 1A Application for Biohaz Agent or rDNA Research
IBC Form 2A Request for Amendment to Biohaz Agent and rDNA protocol
Lab Cleanout/Closure

From CDC and NIH --
Biosafety in Microbiological and Biomedical Laboratories (BMBL) 4th Edition Answers to detailed questions, for example "exactly what kind of work with Yersinia pestis requires BSL3 containment?"

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http://www.chem.purdue.edu/safety | send comments to: swihart@purdue.edu | last review/update August 05, 2010