first: Henry
last: Chou
dept: Chemistry
workrooms: WTHR121
email: hchou@purdue.edu
staffclass: Undergraduate student
jobtitle: Lab Assistant
supervisor: Paul Smith
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.143.89
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; .NET CLR 1.1.4322)
Date: 02/18/2005
Time: 02:11 PM
Measure and prepare reagents, rinse glassware
first: William
last: Fornes
dept: 1215
workrooms: WTHR 101
email: wfornes@purdue.edu
staffclass: A/P Staff
jobtitle: Program Coordinator
supervisor: Gabriela Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Hydrochloric Acid
chem8: Methanol
chem2: Acetone
chem9: Nitric Acid
chem3: Acetonitrile
chem10: Hexane
chem4: Toluene
chem11: Cyclohexane
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.72.83
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1)
Date: 02/18/2005
Time: 03:06 PM
I expect to do very little work with chemicals and am being trained only in case the CASPiE instrumentation manager is unable to be in the lab and it is necessary to transfer waste, replenish mobile phase solvents, etc.
first: Harry
last: Morrison
dept: Chemistry
workrooms: WTHR 430
email: hmorrison@purdue.edu
staffclass: Faculty
jobtitle: Professor
supervisor: Tim Zwier
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: supervisor
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.142.51
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax)
Date: 02/18/2005
Time: 04:04 PM
None
first: Rand
last: Jean
dept: Electrical and Computer Engineering
workrooms: EE350 EE214
email: rjean@purdue.edu
staffclass: Grad student
jobtitle: Research Assistant
supervisor: Dave Janes
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Buffered hydrofluoric acid
chem8: benzene
chem2: electronic grade sulphuric acid
chem9: electronic grade methanol
chem3: electronic grade acetone
chem10: formaldehyde
chem4: chloroform
chem11: 30% hydrogen peroxide
chem5: conc. nitric acid
chem12: conc. sodium hydroxide
chem6: ammonium hydroxide
chem13: OCG 747 negative photoresist
chem7: Nophenol 922
chem14: AZ 1518 positive photoresist
Remote Name: 128.46.209.65
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.0.3705; .NET CLR 1.1.4322)
Date: 02/25/2005
Time: 11:06 AM
1. growing monolayers of organic molecules on metals and semiconductors. 2. etching metals, and oxides off surfaces of semiconductors. 3. characterizing the electrical properties of metal/semiconductor and organic molecules
first: Chidara
last: Sridhar
dept: Chemistry
workrooms: WTHR 463
email: schidara@purdue.edu
staffclass: Postdoctoral
jobtitle: Research Schloar
supervisor: Prof. Sergey N. Savinov
CompletedAndUnderstoodTrainingModule: not answered
InformedOfLabStandardContentsAndLocation: not answered
InformedOfCHPLocationAndAvailability: not answered
InformedOfSafetyInfoLocationAndAvailability: no answer
InformedOfExposureSignsAndSymptoms: no answer
InformedOfPELs: no answer
TrainedAboutMethodsAndObservationsToDetectRelease: no answer
TrainedAboutPhysicalAndHealthHazards: no answer
TrainedAboutProtectiveMeasures: no answer
TrainedAboutCHPDetails: no answer
UnderstandWhomToAskQuestions: no answer
ChemicalList: complete
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.164.171
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1)
Date: 02/25/2005
Time: 06:50 PM
Synthesis of cyclic peptides
first: Sarah
last: Absher
dept: Chemistry
workrooms: 110 WTHR, 101 WTHR
email: sabsher@purdue.edu
staffclass: Undergraduate student
jobtitle: Undergrad research
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1: 30% hydrogen peroxide
chem8: conc. sulfuric acid
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.86.236
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 02/26/2005
Time: 10:25 AM
Lab development for undergraduate chemistry course.
first: Paul
last: Lang
dept: Chemistry
workrooms: WTHR101,110
email: plang@purdue.edu
staffclass: Undergraduate student
jobtitle: Undergrad Research
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.86.194
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 02/26/2005
Time: 10:26 AM
Lab development for undergraduate chemistry course.
first: Meng
last: Zhou
dept: Chemistry
workrooms: WTHR 110
email: zhoum@purdue.edu
staffclass: Undergraduate student
jobtitle: Undergraduate Research CASPiE Pilot
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.86.193
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 02/26/2005
Time: 10:27 AM
Lab development for undergraduate chemistry course
first: Aman
last: Kishore
dept: Chemisty
workrooms: Wthr 101-110
email: aakishor@purdue.edu
staffclass: Undergraduate student
jobtitle: undergrad research
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.86.238
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 02/26/2005
Time: 10:31 AM
lab devolpment for undergrad chemisty course
first: Andrea
last: Cover
dept: Chemistry
workrooms: Weatherill 110 and 101
email: acover@purdue.edu
staffclass: Undergraduate student
jobtitle: undergrad researcher
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.86.237
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 02/26/2005
Time: 10:32 AM
Lab development for undergrad chemistry course.
first: Tiina
last: Kauppila
dept: Chemistry
workrooms: WTHR63
email: tiina.kauppila@helsinki.fi
staffclass: Postdoctoral
jobtitle: visiting post doc
supervisor: R. Graham Cooks
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: methanol
chem8: acetonitrile
chem2: ammonium hydroxide
chem9: ammonium acetate
chem3: formic acid
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.164.32
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 02/28/2005
Time: 10:11 AM
preparation of samples and solvents mass spectrometric experiments
first: Ashley
last: Reiber
dept: chemistry
workrooms: WTHR 101 110
email: areiber@purdue.edu
staffclass: Undergraduate student
jobtitle: researcher
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.164.78
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1)
Date: 03/01/2005
Time: 12:49 PM
Undergraduate Lab Development
first: Melissa L
last: Brink
dept: Chemistry
workrooms: wthr 101, 110
email: mlbrink@purdue.edu
staffclass: Undergraduate student
jobtitle: undergrad research
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.164.78
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.0)
Date: 03/01/2005
Time: 12:49 PM
Undergraduate lab development.
first: Matt
last: Hoch
dept: Chemistry
workrooms: WTHR 101 110
email: mhoch@purdue.edu
staffclass: Undergraduate student
jobtitle: Undergraduate Researcher
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.164.78
Remote User:
HTTP User Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.7.2) Gecko/20040820 Debian/1.7.2-4
Date: 03/01/2005
Time: 12:56 PM
Undergraduate research lab development
first: Haley
last: Goshert
dept: CHM
workrooms: WTHR 101 and 110
email: hgoshert@purdue.edu
staffclass: Undergraduate student
jobtitle: undergraduate researcher
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.86.228
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 03/02/2005
Time: 03:55 PM
Undergraduate Laboratory Development
first: Shannon
last: Borneman
dept: Chemistry
workrooms: WTHR 101 and 110
email: sbornema@purdue.edu
staffclass: Undergraduate student
jobtitle: undergraduate researcher
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.86.233
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 03/02/2005
Time: 03:58 PM
Undergraduate laboratory development
first: KimMarie
last: Barr
dept: Chemistry
workrooms: WTHR 101 110
email: kbarr@purdue.edu
staffclass: Undergraduate student
jobtitle: Undergraduate researcher
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.86.234
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 03/02/2005
Time: 03:59 PM
Undergraduate laboratory developement
first: Aswathy
last: Nair
dept: PULSe
workrooms: Brn bldg
email: aknair@purdue.edu
staffclass: Grad student
jobtitle: graduate student
supervisor: Dr. Kavita Shah
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: will send attachment
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.164.174
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.0.3705)
Date: 03/07/2005
Time: 11:46 AM
Cell Culture work
first: Christian
last: Eichmuller
dept: Chemistry
workrooms: WTHR 345, 333D
email: ceichmul@purdue.edu
staffclass: Postdoctoral
jobtitle: Research Associate
supervisor: Nikolai Skrynnikov
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Ethanol
chem8: 3M aqueous hydrochloride
chem2: 5M aqueous sodium hydroxide
chem9: 0.01% aqueous sodium azide
chem3: phenylmethylsulfonate
chem10: ammoniumperoxodisulfate
chem4: Methanol
chem11: Acetone
chem5: acrylamide
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.142.131
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.1) Gecko/20040707
Date: 03/21/2005
Time: 11:40 AM
Protein expression and purification with E.coli, lab equipment: Shaker, centrifuge, sonicator, FPLC
first: Ning
last: Yin
dept: Chem
workrooms: WTHR 465
email: ningyin@hotmail.com
staffclass: Postdoctoral
jobtitle: Postdoc
supervisor: E. Negishi
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: acetone
chem8: isopropanol sat'd with potassium hydroxide
chem2: hexanes
chem9: ethanol
chem3: ethyl acetate
chem10: chloroform
chem4: dichloromethane
chem11: dimethylformamide
chem5: methanol
chem12: acetonitrile
chem6: tetrahydrafuran
chem13: 1M aqueous hydrochloric acid
chem7: diethyl ether
chem14: pentane
Remote Name: 12.210.186.103
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 03/21/2005
Time: 10:53 PM
prepare compounds using organic reagents and metal reageants
first: Melissa
last: Wilson
dept: Chemistry
workrooms: WTHR 101 110
email: wilson48@purdue.edu
staffclass: Undergraduate student
jobtitle: undergrad researcher
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.164.158
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 03/23/2005
Time: 04:57 PM
Undergraduate Lab development.
first: Paul
last: Smith
dept: Chemistry
workrooms: 121, 162 WTHR
email: psmith4@purdue.edu
staffclass: A/P Staff
jobtitle: Director of Lecture Demonstratons
supervisor: Kurt Keyes
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Concentrate nitric acid
chem8: 6M sodium hydroxide
chem2: Concentrate hydrochloric acid
chem9: 6M postassium hydroxide
chem3: Concentrate Sulfuric acid
chem10: Concentrate ammonium hydroxide
chem4: Bromine neat
chem11: Glacial acetic acid
chem5: Nitric oxide gas
chem12:
chem6: Chlorine gas
chem13:
chem7:
chem14:
Remote Name: 128.210.143.89
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; .NET CLR 1.1.4322)
Date: 03/29/2005
Time: 10:44 AM
To set up, create and present chemistry demonstrations.
first: Matthew
last: Clifton
dept: Biology
workrooms: Lilly B203, B201, B408, B409
email: cliftomc@purdue.edu
staffclass: Grad student
jobtitle: Graduate Student
supervisor: Stauffacher
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: ethanol
chem8: Vanadate
chem2: acetone
chem9: AMPPNP
chem3: nickle sulfate
chem10: D-Ribose-C14 (1st position)
chem4: acrylamide
chem11:
chem5: TEMED
chem12:
chem6: coomassie blue
chem13:
chem7: EDTA
chem14:
Remote Name: 128.210.18.164
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.6) Gecko/20050317 Firefox/1.0.2
Date: 03/29/2005
Time: 12:52 PM
I grow bacterial cells and force them to overexpress membrane proteins. These proteins are purified by Ni-NTA chromatography and the purity is checked by SDS-PAGE analysis. These samples are further examined using analytical ultracentrifugation. Soon I will test the binding of C14 Ribose to these proteins.
first: Adam
last: Zabell
dept: BIOL
workrooms: LILY B-408,409,201,202,203
email: zabell@purdue.edu
staffclass: Postdoctoral
jobtitle: Postdoctoral Fellow
supervisor: Cynthia Stauffacher
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Ethanol
chem8: Methanol
chem2: Isopropanol
chem9: dry ice
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.48.217
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X; en) AppleWebKit/125.5.7 (KHTML, like Gecko) Safari/125.12
Date: 03/29/2005
Time: 03:44 PM
I grow E coli in typical media conditions, purify grown protein with simple and dilute acidic solutions. I do protein crystallization for X-ray structure determination, and also do enzyme kinetics that is monitored by UV/vis spectrometry.
first: xinqi
last: liu
dept: biological sciences
workrooms: lily hall, B321
email: liu13@purdue.edu
staffclass: A/P Staff
jobtitle: Assistant research scientist
supervisor: Richard J. Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: acetone
chem8: acetonitrile
chem2: EtOH
chem9: NaOH
chem3: EtOAc
chem10: KOH
chem4: Formaldehyde
chem11: HCl
chem5: MeOH
chem12: isopropanol
chem6: chloroform
chem13: hexane
chem7: pyridine
chem14: phenol
Remote Name: 128.210.18.204
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7) Gecko/20040616
Date: 03/29/2005
Time: 03:49 PM
chemicals: EtOH, MeOH, NaOH, HCl, Tris, NaH2PO4, etc. instruments: centrifuge, FPLC, pH meter, etc.
first: Ranjit
last: Warrier
dept: Biology
workrooms: Lilly B127
email: warrier@purdue.edu
staffclass: Grad student
jobtitle: Grad Student
supervisor: Richard J. Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Ethanol
chem8:
chem2: Chloroform
chem9:
chem3: Phenol
chem10:
chem4: concentrated hydrochloric acid
chem11:
chem5: 1M sodium hydroxide
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.18.203
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.5) Gecko/20041107 Firefox/1.0
Date: 03/30/2005
Time: 11:29 AM
Adding chemicals to 1.5ml and 0.5ml tubes, measuring amounts of reagents to mix in 1L beakers
first: Cynthia V
last: Stauffacher
dept: Biological Sciences
workrooms: Lilly B-406B, 408, 404, 201, 202, 203, 204, 102 141
email: cstauffa@purdue.edu
staffclass: Faculty
jobtitle: Professor
supervisor: Cynthia V Stauffacher
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: supervisor
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.18.160
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 5.14; Mac_PowerPC)
Date: 03/30/2005
Time: 11:42 AM
Although I am the research director in my laboratory, I do some of the experiments on the equipment used to produce the X-ray crystallographic data, including high power X-ray generators and X-ray detectors.
first: Bong-Suk
last: Kim
dept: Biology
workrooms: B-127, Lilly Hall
email: Kim1@purdue.edu
staffclass: Postdoctoral
jobtitle: Postdoctoral
supervisor: Richard J. Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: 70% Ethyl alcohol
chem8: Acetic acid
chem2: Ethidium Bromide
chem9: Phenol
chem3: Sodium hydoxide
chem10:
chem4: Hydrocholoric acid
chem11:
chem5: Sodium dodesyl sulfate
chem12:
chem6: Acetone
chem13:
chem7: Methy alcohol
chem14:
Remote Name: 128.210.18.151
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X; en) AppleWebKit/125.2.2 (KHTML, like Gecko) Safari/125.7
Date: 03/30/2005
Time: 12:35 PM
I am a molecular biologist and work on virus. I do lots of DNA cloning and RNA works. Also, I purify protein, virus and nucleic acids from cells.
first: Kathryn
last: Zabell
dept: Biology
workrooms: Lilly: B408, B409, B201, B204
email: durfee@purdue.edu
staffclass: Grad student
jobtitle: Grad Student
supervisor: Cynthia V. Stauffacher
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: hydrochloric acid
chem8: glacial acetic acid
chem2: ethanol
chem9: 1 M aqueous imidazole
chem3: Potassium hydroxide pellets
chem10: Sodium hydroxide pellets
chem4: Sodium dodecyl sultate powder
chem11: acetone
chem5: 10 mg/ml aqueous Ethidium Bromide
chem12: Tris/Glycine Buffer
chem6: Phenol
chem13: Methanol
chem7: Kanamycin sulfate
chem14: Tetracycline
Remote Name: 128.210.18.80
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.5) Gecko/20041107 Firefox/1.0
Date: 03/30/2005
Time: 01:42 PM
Assemble and run polymerase chain reactions (PCR) using chemicals, buffers, and a thermocycler. Make and run both agarose and SDS gels for sample anaylsis using horizontal and vertical electrophoresis units, chemicals, buffers, and stains. Bacterial cell transformation and protein expression using chemicals, buffers, an incubator/shaker, water baths, and superspeed centrifuges. Protein purification using chemicals, buffers, affinity chromatography, proteolysis, dialysis, and sample concentrators. Protein phosphorylation and interaction determination using Western blot, electrophoresis, antibodies, and film development (automatic developer). X-ray crystallography using various chemicals, buffers, crystal trays, rotating anode x-ray generator, and liquid nitrogen for freezing crystals.
first: Dagmar
last: Sedlak
dept: Biol.Sci.
workrooms: Lilly B 127,143, 227, 321, Biochm P3 area
email: sedlakd@purdue.edu
staffclass: A/P Staff
jobtitle: Lab Manager
supervisor: Richard J Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Chloroform
chem8: sodium hydroxide
chem2: phenol
chem9: ethidium bromide
chem3: methanol
chem10: sodium dodecyl sulfate
chem4: ethanol
chem11: acrylamide
chem5: Acetone
chem12: dimethylsulfoxide
chem6: acetic acid
chem13:
chem7: hydrochloric acid
chem14:
Remote Name: 128.210.18.126
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X; en) AppleWebKit/125.5.5 (KHTML, like Gecko) Safari/125.11
Date: 03/30/2005
Time: 02:28 PM
performing experiments, making solutions
first: Chinmay
last: Patkar
dept: Biological Sciences
workrooms: Lilly B-127
email: patkarc@purdue.edu
staffclass: Grad student
jobtitle: Grad Student
supervisor: Richard J. Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Ethanol
chem8:
chem2: Chloroform
chem9:
chem3: Phenol
chem10:
chem4: concentrated hydrochloric acid
chem11:
chem5: 1M sodium hydroxide
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.18.209
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax)
Date: 03/30/2005
Time: 03:10 PM
Adding chemicals to small 0.5mL and 1.5mL tubes. Measuring and mixing reagents in 1L flasks and beakers to make buffers and media.
first: clara
last: santato
dept: chemistry
workrooms: 5128-5130-5130b
email: csantato@purdue.edu
staffclass: Postdoctoral
jobtitle: Dr
supervisor: Choi
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: tin dichloride
chem8:
chem2: dmso
chem9:
chem3: sodium nitrate
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.142.117
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; MathPlayer 2.0)
Date: 03/31/2005
Time: 01:48 PM
preparation by electrodeposition in non aqueous media (DMSO) of tin dioxide nanostructures for photoelectrochemical purposes
first: Joshua
last: Robertson
dept: BIOL
workrooms: Lilly G-234
email: robert29@purdue.edu
staffclass: Undergraduate student
jobtitle: Undergraduate Researcher
supervisor: Dr. Ignacio Camarillo
CompletedAndUnderstoodTrainingModule: no
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Methanol
chem8:
chem2: Chloroform
chem9:
chem3: Acetic Acid Glacial
chem10:
chem4: Ethanol
chem11:
chem5: 2-mercaptoethanol
chem12:
chem6: acrylamide
chem13:
chem7: temed
chem14:
Remote Name: 68.58.122.186
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 04/02/2005
Time: 05:45 PM
Grow and maintain MCF-7 cancer cells to analyze intracellular protein levels associated with breast cancer using electrophoresis.
first: Mary
last: Sever
dept: Chemistry
workrooms: 4125, 4125A, 4135, 4135A
email: sever@purdue.edu
staffclass: Grad student
jobtitle: reasearch assistant
supervisor: Wilker
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: dichloromethane
chem8: methanol
chem2: tetrabutylammonium fluoride
chem9: trifluoroacetic acid
chem3: dimethyl formamide
chem10: acetonitrile
chem4: chromium(III) acetate
chem11: sodium hydroxide in water (various molarities)
chem5: manganese(III) acetate
chem12: Alanine-DOPA-Threonine-proline peptides
chem6: piperidine
chem13: catechol
chem7: 3,4-dihydroxyphenylalanine
chem14: argon
Remote Name: 128.211.173.108
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 5.23; Mac_PowerPC)
Date: 04/04/2005
Time: 10:39 AM
Synthesis and column purification of the protected DOPA derivative, Fmoc-DOPA(TBDMS)2 . Solid phase peptide synthesis of the 4 amino acid peptide alanine-DOPA-threonine-proline. Deprotection and clevage of the peptide from the solid support. High pressure liquid chromatography purification of peptides. Base titrations of peptide-metal ion solution to produce various metal-peptide complexes.
first: Donna
last: Fekete
dept: Biological Sciences
workrooms: LILY 2-122, 2-218, 2-219, 2-228, 2-229
email: dfekete@purdue.edu
staffclass: Faculty
jobtitle: Associate Professor
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: supervisor
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.89.116
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.5) Gecko/20041107 Firefox/1.0
Date: 04/04/2005
Time: 04:35 PM
I make solutions using chemicals that may be flammable (eg. ethanols), hazardous (eg., Xylenes), toxic (eg., paraformaldehyde), usually in relatively small quantities (one liter volumes) and rather dilute solutions (biologically compatible with tissue samples). I use a cryostat and store tissue samples in liquid nitrogen or -80oC freezers. I use the autoclave. I use open flames on the bench for sterilization. I heat solutions, usually no hotter than 65oC on the bench or in waterbaths.
first: Tripti
last: Bera
dept: Biological Sciences
workrooms: Lilly B-321
email: tbera@bilbo.bio.purdue.edu
staffclass: Technical
jobtitle: Lab Technician
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Ethanol
chem8:
chem2: Methanol
chem9:
chem3: Chloroform
chem10:
chem4: Phenol
chem11:
chem5: conc. hydrochloric acid
chem12:
chem6: sodium hydroxide
chem13:
chem7: acrylamide
chem14:
Remote Name: 128.210.18.209
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax)
Date: 04/05/2005
Time: 02:26 PM
Mixing reagents to make solutions like buffers and bacterial media.
first: Muriel
last: Soler
dept: Biological Sciences
workrooms: Lilly B127, B321
email: msoler@purdue.edu
staffclass: Postdoctoral
jobtitle: research associate
supervisor: Richard J. Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: chloroform
chem8:
chem2: phenol
chem9:
chem3: 1M sodium hydroxide
chem10:
chem4: 1M acetic acid
chem11:
chem5: 1M chloridic acid
chem12:
chem6: ethidium bromide
chem13:
chem7:
chem14:
Remote Name: 128.210.18.155
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X; en) AppleWebKit/125.4 (KHTML, like Gecko) Safari/125
Date: 04/06/2005
Time: 01:01 PM
I'm using chemicals for molecular biology's experiments like DNA purification or Dna fragments migration on a gel containing ethidium bromide for UV visualization.
first: Aloke
last: Bera
dept: Biology
workrooms: B-321. Lilly Hall
email: bera@purdue.edu
staffclass: Postdoctoral
jobtitle: Research Associate
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Ethanol
chem8: Acetone
chem2: Methanol
chem9: Acetic Acid
chem3: Acrylamide
chem10: Isopropyl Alcohol
chem4: Chloroform
chem11:
chem5: Phenol
chem12:
chem6: Conc HCL
chem13:
chem7: Sodium hygroxide
chem14:
Remote Name: 128.210.18.230
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.0)
Date: 04/07/2005
Time: 09:02 AM
Make buffer,media,staining solution,gel preparation
first: Moumita
last: Sen
dept: Biology
workrooms: Lilli B-408, B-201, B-203
email: senm@purdue.edu
staffclass: Grad student
jobtitle: Graduate Research Assistant
supervisor: Dr. Cynthia Stauffacher
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Acrylamide
chem8: Ethanol
chem2: Tris
chem9: Methanol
chem3: Dithiothreitol
chem10: EDTA
chem4: Sodium hydroxide
chem11: Sodium dodecyl sulfate
chem5: Hydrochloric acid
chem12: TEMED
chem6: Glacial acetic acid
chem13: Ammonium persulfate
chem7: Acetone
chem14: Isopropanol
Remote Name: 128.210.18.212
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 04/07/2005
Time: 12:40 PM
Protein purification, which involves handling buffers, chromatography columns, denaturing SDS polyacrylamide gels for proteins, the French press; setting up protein crystallization that involves mixing protein with different solutions of different buffer, salt and organic compound compositions.
first: Yu-hsuan
last: Chang
dept: Biological Sciences
workrooms: LILY, B-127
email: chang23@purdue.edu
staffclass: Grad student
jobtitle: RA
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Ethidium Bromide
chem8: acrylamide
chem2: Ethanol
chem9: sodium dodecyltsulfate
chem3: Methanol
chem10: dimethyl sulfoxide
chem4: Acetic acid
chem11: phenol
chem5: 10M aqueous hydrochloride
chem12: choloroform
chem6: Sulfuric acid
chem13: Sodium hydroxide
chem7: Ammonium persulfate
chem14: 10M aqueous potassium hydroxide
Remote Name: 128.210.18.233
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1)
Date: 04/07/2005
Time: 03:44 PM
All the reagents are applied to DNA and protein work.
first: Eunmee
last: Hong
dept: BIOL
workrooms: B127 Lilly
email: ehong@purdue.edu
staffclass: Grad student
jobtitle: graduate research assistant
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: acrylamide
chem8: ethidium bromide
chem2: phenol
chem9: acetic acid
chem3: chloroform
chem10: sodium dodesyl sulfate
chem4: ethanol
chem11: dimethyl sulfoxide
chem5: methanol
chem12: sulfuric acid
chem6: hydrochloric acid
chem13: sodium hydroxide
chem7: ammonium persulfate
chem14: potasium hydroxide
Remote Name: 128.210.18.154
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 5.21; Mac_PowerPC)
Date: 04/07/2005
Time: 03:46 PM
We generally use polyacrylamide gels for the analysis of proteins. Starting with 30% of acrylamide gel stock which contains 30% acrylamide and 1% of bis-acrylamide, we adjust the final concentration of acrylamide and add ammonium persulfate and TEMED for solidifying reagents just before we pour the gel to the gel apparatus.
first: Chanakha
last: Navaratnarajah
dept: Biology
workrooms: Lilly B-127
email: navaratn@purdue.edu
staffclass: Grad student
jobtitle: Graduate Research Assistant
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: ethanol
chem8: chloroform
chem2: hydrochloric acid
chem9: phenol
chem3: ethidium bromide
chem10: potassium hydroxide
chem4: sodium hydroxide
chem11: dimethyl sulfoxide
chem5: sulfuric acid
chem12: acrylamide
chem6: phosphoric acid
chem13: N,N,N',N'-Tetramethylethylenediamine
chem7: acetone
chem14: nitric acid
Remote Name: 128.210.18.244
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax)
Date: 04/07/2005
Time: 04:14 PM
Cell culture Cloning Protein Purification
first: Joyce
last: Jose
dept: Biology
workrooms: Lilly B127
email: jjose@purdue.edu
staffclass: Postdoctoral
jobtitle: Postdoctoral
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Phenol
chem8: Ethanol
chem2: Ethidium bromide
chem9: Hydrochloric acid
chem3: Chloroform
chem10: Sulfurica acid
chem4: Isopropanol
chem11: Sodium hydroxide
chem5: Isoamylalcohol
chem12: Acetic acid
chem6: Formaldehyde
chem13: Acrylamide
chem7: Methanol
chem14: Sodium dodecyl sulphate
Remote Name: 128.210.18.244
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax)
Date: 04/07/2005
Time: 04:39 PM
Cloning Cell culture Protein purification
first: Mayuri
last: Mayuri
dept: Biology
workrooms: Lilly, B127,B-227
email: mmayuri@purdue.edu
staffclass: Grad student
jobtitle: Graduate student
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Phenol
chem8:
chem2: Chloroform
chem9:
chem3: SDS
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.18.90
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.0.3705)
Date: 04/07/2005
Time: 04:40 PM
Molecular biology work for cloning purposes and protein biochemistry.
first: Ulrike J.
last: Sienknecht
dept: Bio Sciences
workrooms: 2-122, 2-228
email: Sienknecht@purdue.edu
staffclass: Postdoctoral
jobtitle: Postdoc
supervisor: Dr. Fekete
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: does not apply
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Paraformaldehyde
chem8: Formamide
chem2: Methanol
chem9: Chlorophorm
chem3: Ethanol
chem10: Phenol
chem4: Isopropylalcohol
chem11: Acetone
chem5: Ethidiumbromid
chem12: Sodium Azide
chem6: DEPC
chem13:
chem7: Lithium Chloride
chem14:
Remote Name: 128.211.168.200
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X; de-de) AppleWebKit/125.5.7 (KHTML, like Gecko) Safari/125.12
Date: 04/07/2005
Time: 05:18 PM
processing embryonic tissue for histology and molecular applications
first: Elisa
last: La Bauve
dept: Biological Sciences
workrooms: Lily B321 and B127, Biochem P3
email: elabauve@purdue.edu
staffclass: Grad student
jobtitle: Graduate Research Assistant
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Phenol
chem8:
chem2: Chloroform
chem9:
chem3: Isoamyl Alcohol
chem10:
chem4: Ethidium Bromide
chem11:
chem5: Methanol
chem12:
chem6: Acrylimde
chem13:
chem7:
chem14:
Remote Name: 128.210.18.98
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X; en) AppleWebKit/124 (KHTML, like Gecko) Safari/125.1
Date: 04/07/2005
Time: 05:31 PM
In isolation and purification of DNA, I use phenol, chloroform, isoamyl alcohol. In visualizaition of protein and membrane transfer, I use methanol and acrylimide. I also use methanol as a permeabilizing agent in cell culture.
first: Jessica
last: Verburg
dept: Biology
workrooms: Lilly 2-205, 2-206, 2-238
email: jverburg@purdue.edu
staffclass: Grad student
jobtitle: Graduate Student
supervisor: Dr. Peter Hollenbeck
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.89.187
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.0.2) Gecko/20030208 Netscape/7.02
Date: 04/07/2005
Time: 05:45 PM
none
first: Karla Ann
last: Combs
dept: Biology
workrooms: B321, B127, B227 Lilly Hall
email: kacombs@purdue.edu
staffclass: Grad student
jobtitle: graduate student
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Ethanol
chem8: phenol
chem2: chloroform
chem9: methanol
chem3: isopropanol saturated with cesium chloride
chem10: acrylamide
chem4: ethidium bromide
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.18.201
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.0)
Date: 04/07/2005
Time: 10:56 PM
As a biologist, I work with ethanol to disinfect surfaces and also to precipitate DNA. Phenol, chloroform and an isopropanol solution saturated with cesium chloride are also used in DNA preparation. Several gel types are used in the lab: agarose and acrylamide. Acrylamide is the more dangerous of the two. In powder form we use a mask to prevent inhalation of acrylamide. In the destaining solution to visualize bands we use methanol. However in the other gel type, agarose, we use ethidium bromide to visualize nucleic acid. The lab equipment I use most are centrifuges, gel running apparatuses, cell hoods, and pipettes.
first: Takunori
last: Satoh
dept: Biological Sciences
workrooms: LILY 2-122
email: tsatch@bilbo.bio.purdue.edu
staffclass: Postdoctoral
jobtitle: Research Associate
supervisor: Donna Fekete
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Paraformaldehyde
chem8: Ethanol
chem2: Isopropanol
chem9: Methanol
chem3: Diaminobensidine (DAB)
chem10: Aceton
chem4: Triethanolamine
chem11: Acetic anhydride
chem5: bisbenzamide (Hoechst)
chem12: glutaraldehyde
chem6: Nitroblue tetrazolium (NBT)
chem13: 5-Bromo-4-chloro-3-indolyl Phosphate (BCIP)
chem7: Sodium Azide
chem14: Dimethyl sulfoxide
Remote Name: 128.210.89.135
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X; ja-jp) AppleWebKit/125.5.6 (KHTML, like Gecko) Safari/125.12
Date: 04/08/2005
Time: 12:11 PM
1. Fixation of tissue using Paraformaldehyde or glutaraldehyde in PBS. 2. Dehydrate tissues using Ethanol, Methanol or Aceton 3. Precipitate DNA with Ethanol or Isopropanol 4. Stain tissue sections with DAB, NBT, BCIP and/or Hoechst 5. Use sodium azide as preservative of antibody. 6. Use Dimethyl sulfoxide as solvent of NBT and BCIP. 7. Use Ultracentrifuge to concentrate virus 8. Use 1% acetic anhydride in 0.1M triethanolamine / water to acetylate tissue sections.
first: calvin
last: steussy
dept: Biology
workrooms: Lilly B203
email: csteussy@purdue.edu
staffclass: Postdoctoral
jobtitle: post doctoral research associate
supervisor: Cyntha Stauffacher
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: sodium hydroxide, solid
chem8: concentrated hydrochloric acid
chem2: liquid nitrogen
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.48.230
Remote User:
HTTP User Agent: Mozilla/5.0 (X11; U; Linux x86_64; en-US; rv:1.7.5) Gecko/20041107 Firefox/1.0
Date: 04/08/2005
Time: 12:44 PM
cloning dna into bacterial cells and inducing those cells to overexpress the protein coded in the dna. These cells are broken and the overexpressed protein extracted and purified. the pure protein is then crystalized by gentle dehydration. the crystal is frozen in liquid nitrogen and studied with high energy x-rays.
first: Etti
last: Harms-Van Etten
dept: Biology
workrooms: Lilly, B321,B316, B318
email: eharms@purdue.edu
staffclass: A/P Staff
jobtitle: Assoc.Res.Scientist
supervisor: Prof. R. Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Ethidium Bromide
chem8: Dodecyl Sodium Sulfate
chem2: Phenol
chem9: Acetone
chem3: Chloroform
chem10: Sodium Hydroxide
chem4: Ethanol
chem11: Potassium Hydroxide
chem5: Methanol
chem12: iso-Propanol
chem6: Acetic Acid
chem13: Hydrochloric Acid
chem7: Polyacrylamide
chem14: Sodium Hydroxide
Remote Name: 128.210.18.189
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 5.23; Mac_PowerPC)
Date: 04/08/2005
Time: 12:50 PM
Making buffers, staining and destaining solutions, making acylamide gels, agarose gels containing ethidium bromide, desinfect areas with ethanol, visualizing DNA/RNA with UV-light
first: Andrea
last: Campero
dept: Biology
workrooms: Lilly 2-122
email: acampero@hotmail.com
staffclass: Grad student
jobtitle: graduate student
supervisor: Donna Fekete
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: ethanol
chem8: methanol
chem2: paraformaldehyde (4% in phosphate buffered saline)
chem9:
chem3: diamino benzene
chem10:
chem4: 10% sodium azide
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.211.168.26
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.0.3705)
Date: 04/08/2005
Time: 01:35 PM
solution preparation and slide staining
first: Anthony
last: Battisti
dept: Bio. Sci.
workrooms: Lilly B102, B216, B421, B422
email: battisti@bilbo.bio.purdue.edu
staffclass: A/P Staff
jobtitle: Electron Microscopy Assistant
supervisor: Paul Chipman
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: liquid nitrogen
chem8:
chem2: liquid ethane
chem9:
chem3: uranyl acetate
chem10:
chem4: ethylene dicholoride
chem11:
chem5: compressed nitrogen
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.18.229
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 5.23; Mac_PowerPC)
Date: 04/08/2005
Time: 03:58 PM
Preparation of concentrated viral particles samples for cryo or negative stain transmission electron microscopy. Negative stain samples require uranyl acetate to be applied to EM grids. Cryo-EM requires plunge-freezing in liquid ethane and storage in liquid nitrogen. Preparation of carbon coated grids requires ethylene dichloride for production and removal of formvar layer (a plastic which is coated with carbon).
first: Paul
last: Chipman
dept: Biol. Sci.
workrooms: Lilly B216, B421, B422, B423
email: paulrc@purdue.edu
staffclass: A/P Staff
jobtitle: Electron Microscopy Facility Manager
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: liquid nitrogen
chem8: uranyl acetate
chem2: liquid ethane
chem9: ethylene dichloride
chem3: compressed gases
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.155.233
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.3) Gecko/20040910
Date: 04/08/2005
Time: 04:20 PM
sample preparation for electron microscopy
first: mandana
last: amiri
dept: Biological Sciences
workrooms: 2-205&311- Lilly hall
email: mamiri@purdue.edu
staffclass: Grad student
jobtitle: graduate teaching assistant
supervisor: Dr. Hollenbeck
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: ethanol (70%)
chem8: 1:50 dilution of fluorescin-tyramide solution (fluorescent dye from Perkin- Elmer kit
chem2: paraformaldehyde (4%)
chem9:
chem3: 1 M aqueous hydrochloric acid
chem10:
chem4: 1M aqueous sodium hydroxide
chem11:
chem5: 0.1M aqueous Boric Acid
chem12:
chem6: (.03%) aqueous Triton x
chem13:
chem7: 100 nM aqueous Mitotracker fluorescent dye
chem14:
Remote Name: 128.210.89.187
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; (R1 1.3))
Date: 04/11/2005
Time: 12:39 PM
I use acid and bases mainly to buffer the solutions. The rest of the chemicals are for In situ hybridization work. They are used for fixing, washing cultured cells and detecting mitochondrial DNA under the microscope.
first: Shailly
last: Tomar
dept: Biology
workrooms: B321 - Lilly
email: stomar@purdue.edu
staffclass: Grad student
jobtitle: Research Assistant
supervisor: Richard J. Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Ethanol
chem8: Conc. HCl
chem2: Acetic Acid
chem9: Phenol
chem3: Sodium dodecyl Sulfate
chem10: P32 Radioactive Material
chem4: Liquid Nitrogen
chem11: Methanol
chem5: Acrylamide
chem12: DIETHYL PYROCARBONATE (DEPC)
chem6: Chloroform
chem13: Phenylmethylsulphonylfluoride [PMSF]
chem7: 10M NaOH
chem14: Tween-20
Remote Name: 128.210.18.75
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.7.3) Gecko/20040910
Date: 04/11/2005
Time: 06:48 PM
I express protein in Bacteria and purify it, run it on polyacrlyamide (PAGE) gels and try and crystallize it to look at its structure. Using this purified protein i do assays to see if protein has enzymatic activity...for this i use radioactive P32.
first: valorie d
last: bowman
dept: Biology
workrooms: Lilly B421
email: vdb@purdue.edu
staffclass: A/P Staff
jobtitle: Senior Research Electron Microscopist
supervisor: Paul Chipman
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Liquid Nitrogen
chem8: Ethanol 70%
chem2: Acetone
chem9: Ethane gas
chem3: Uranyl Acetate powder
chem10: Liquid Ethane
chem4: Dichloroethane
chem11:
chem5: Chloroform
chem12:
chem6: Sodiumhydroxide 1M
chem13:
chem7: Uranyl Acetate 1% solution
chem14:
Remote Name: 128.210.18.100
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 5.16; Mac_PowerPC)
Date: 04/12/2005
Time: 09:51 AM
I freeze small aliquots of virus suspensions (non- human pathogens) mounted on EM grids by quickly plunging them into liquid ethane. I then transfer them into liquid nitrogen for storage or insertion into the Electron microscope.
first: Albert
last: Urazaev
dept: Biological Sciences
workrooms: Lilly B320, B317
email: aurazaev@purdue.com
staffclass: A/P Staff
jobtitle: Assistant Research Scientist
supervisor: Christie Sahley
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: does not apply
InformedOfPELs: does not apply
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: parafirmaldehyde
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.18.239
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; (R1 1.5); .NET CLR 1.1.4322)
Date: 04/12/2005
Time: 01:26 PM
I do electrophysiological, behavioral and morphological experiments using leeches and their nervous system. This includes microelectrode and suction electrode recordings, microelectrode injections of dyes in single cells, confocal microscopy. The chemicals I deal with are mostly salts, intracellular dyes (Lucifer Yellow, Rhodamine Dextran), ethanol, serotonin receptors agonists and antagonists (serotonin, pimozide), fixatives (paraformaldehyde, glycerol).
first: Ranjan
last: Sengupta
dept: Biological sciences
workrooms: B-321-Lily hall
email: rsengupt@purdue.edu
staffclass: Grad student
jobtitle: Research assistant
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Phenol
chem8: Amonium persulfate
chem2: Acetone
chem9: hydrochloric acid
chem3: Methanol
chem10: nitric acid
chem4: Acetic acid
chem11: sulphuric acid
chem5: Chloroform
chem12: Ethidium Bromide
chem6: Sodium Dodecyl sulphate
chem13: Bleach
chem7: Isopropanol
chem14: Polyacrylamide
Remote Name: 128.210.18.248
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X; en) AppleWebKit/125.5.7 (KHTML, like Gecko) Safari/125.12
Date: 04/12/2005
Time: 03:17 PM
I work in a molecular biology and protein purification lab. DNA large prep isolations and protein purification, electrophoresis, gel staining, are used routinely.
first: Elizabeth E
last: Hamilton
dept: Chemistry
workrooms: BRWN 4125A
email: hamiltee@purdue.edu
staffclass: Grad student
jobtitle: Teaching assistant
supervisor: Jonathan Wilker
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Acetone
chem8: Ethanol
chem2: Diethyl sulfate
chem9: Ethyl iodide
chem3: Ethyltrifluormethanesulfonate
chem10: Ethidium bromide
chem4: Acetonitrile
chem11: Sodium orthovanadate
chem5: Sodium selenate
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.211.174.184
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X; en-us) AppleWebKit/125.5.7 (KHTML, like Gecko) Safari/125.12
Date: 04/12/2005
Time: 05:40 PM
I synthesize inorganic compounds (primarily vanadates). I use NMR to monitor reactions between oxoanions (such as vanadates, selenates, phosphates) and alkylating agents (such as diethyl sulfate and ethyl iodide).
first: Cecilia Y
last: Martinez
dept: Biological Sciences
workrooms: Lilly 2-206 and Lilly 2-311
email: cmartin@bilbo.bio.purdue.edu
staffclass: Grad student
jobtitle: graduate research assistant
supervisor: Peter J. Hollenbeck
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1:
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Remote Name: 128.210.89.182
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1)
Date: 04/15/2005
Time: 06:22 PM
none
first: Andre
last: Venter
dept: Chemistry
workrooms: WHRL 63, 65
email: aventer@purdue.edu
staffclass: Postdoctoral
jobtitle: Researcher
supervisor: R.G.Cooks
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: acetonitrile
chem8: various aminoacids
chem2: methanol
chem9:
chem3: formic acid
chem10:
chem4: acetic acid
chem11:
chem5: propanoic acid
chem12:
chem6: sodium hydroxide
chem13:
chem7: sodium bicarbonate
chem14:
Remote Name: 128.210.164.94
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1)
Date: 04/18/2005
Time: 03:00 PM
mass spectrometry using electrospray ionization of samples containing trace amounts of chemicals dissolved in methanol water or acetonitrile water solutions.
first: xuehua
last: li
dept: chemistry
workrooms: WTHR 440
email: li85@purdue.edu
staffclass: Visiting
jobtitle: professional
supervisor: Alexander Wei
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Chloroform
chem8: Ethyl Ether
chem2: Acetone
chem9: Toluene
chem3: Benzene
chem10: Hexanes
chem4: Carbon Disulfide
chem11: Tetrahydrofuran
chem5: Ethyl Acetate
chem12: Acetic Acid
chem6: Methanol
chem13: Acetonitrile
chem7: Pyridine
chem14: Isopropanol
Remote Name: 128.210.187.15
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.0)
Date: 04/18/2005
Time: 07:55 PM
Mainly in organic synthesis, such as weighting, adding and transfering chemical ragents; To collect and seperate reaction product by distilling, filting, recrystalling, extracting, TLC,etc. To use instrument, such as NMR, IR, UV-vis etc, to characterize the product.
first: Jingwei
last: Li
dept: PULSe
workrooms: lily-212
email: li62@purdue.edu
staffclass: Grad student
jobtitle: rotation student
supervisor: donna fekete
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: acetone
chem8:
chem2: EtOH
chem9:
chem3: formaldehyde
chem10:
chem4: bezene
chem11:
chem5: palloidin
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.211.201.172
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1)
Date: 04/19/2005
Time: 11:18 AM
i use palloidin to stain chick embryo sections. since now i am just rotating,i did not do much work
first: Dheeraj
last: Khare
dept: Biological Sciences
workrooms: B117,B118 (LILY)
email: dkhare@purdue.edu
staffclass: Postdoctoral
jobtitle: postdoctoral research associate
supervisor: Jue Chen
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: 0.1 M Hydrogen chloride
chem8: Formaldehyde
chem2: 10 M Sodium hydroxide
chem9: 30 % Acrylamide/Bis
chem3: Ethanol
chem10: N,N,N',N'-Tetramethylethylenediamine
chem4: Methanol
chem11: 5 M Sodium Chloride
chem5: Acetic acid
chem12: Potassium hydroxide
chem6: 10% Sodium dodecylsulfate
chem13: 50% Polyethylene glycol 4000
chem7: 2-mercapto ethanol
chem14: 50% Polyethylene glycol 400
Remote Name: 128.210.48.67
Remote User:
HTTP User Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.6) Gecko/20040413 Debian/1.6-5
Date: 04/19/2005
Time: 03:21 PM
My work involves crystal structure determination of maltose transporter using X-rays. For this we need highly pure protein samples. The gene expressing the desired protein is overexpressed in E coli. The bacterial cells are later harvested and lysed using French Pressure cell. The membrane fraction is washed and the protein is solubilized using a detergent. For protein purification affinity tags are used and followed by ion exchange or size exclusion chromatograhy.
first: Peter
last: Hollenbeck
dept: BIOL
workrooms: LILY 2-206, 2-311
email: phollenb@purdue.edu
staffclass: Faculty
jobtitle: Professor
supervisor: Richard Kuhn
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: 1 M aqueous urea
chem8:
chem2: ethanol
chem9:
chem3: formalin
chem10:
chem4: 1 M aqueous sodium hydroxide
chem11:
chem5: 1 M aqueous potassium hydroxide
chem12:
chem6: isopropanol
chem13:
chem7:
chem14:
Remote Name: 128.210.89.179
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.6) Gecko/20050317 Firefox/1.0.2
Date: 04/19/2005
Time: 03:34 PM
I apply small amounts of fixatives and solvents to cell cultures to prepare them for microscopy. I use small amounts of caustic agents and solvents to prepare DNA samples from bacterial cultures.
first: Barbara
last: Damsz
dept: Biology
workrooms: Hansen Bldg Rm 215A
email: bdamsz@bilbo.bio.purdue.edu
staffclass: A/P Staff
jobtitle: Laboratory Manager
supervisor: Dr. Stephen F. Konieczny
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Acetone
chem8: Formaldehyde
chem2: Bleach
chem9: Glutaraldehyde
chem3: Butyl Alcohol
chem10: Hydrochloric Acid
chem4: Cesium Chloride
chem11: Liquid Nitrogen
chem5: Chloroform
chem12: Methyl Alcohol
chem6: Ethydium Bromide
chem13: Osmic Acid
chem7: Ethyl Alcohol
chem14: Sodium Dodecyl Sulphate
Remote Name: 128.210.50.100
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1)
Date: 04/19/2005
Time: 04:36 PM
I manage daily operations in two molecular biology labs which includes maintaining lab equipment(CO2 incubators, centrifuges, biosafety cabinets, pH meters and other lab instruments), preparing tissue culture media, solutions and sera, maintaining the labs chemical supplies and disposing of chemical and biohazardous waste.
first: Melissa
last: Wilson
dept: Chemistry
workrooms: WTHR110, 101
email: wilson48@purdue.edu
staffclass: Undergraduate student
jobtitle: Undergrad Researcher
supervisor: Weaver
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
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Remote Name: 128.210.86.205
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 04/20/2005
Time: 06:29 PM
Undergraduate lab development.
first: Brooks
last: Griswold
dept: vet
workrooms: Pharmacy
email: bgriswol@purdue.edu
staffclass: Faculty
jobtitle: Faculty
supervisor: Jean Horner
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Pharmaceutical Products
chem8:
chem2:
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Remote Name: 128.210.96.58
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax)
Date: 04/21/2005
Time: 04:31 PM
Unit dosing of prepared pharmaceutical products. Transfer of products from pharmacy to automated dispensing machine.
first: Gang
last: Lu
dept: Bio.Sci.
workrooms: B-118 Lilly
email: lu31@purdue.edu
staffclass: Grad student
jobtitle: Graduate Student
supervisor: Jue Chen
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: 2-betapercapetoethanol
chem8: choloroform
chem2: ethidium bromide
chem9: 4-aminobenzamidin
chem3: N,N,N',N'-Tetramethylethylenediamine
chem10: 30 % Acrylamide/Bis
chem4: Potassium hydroxide
chem11:
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chem6:
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Remote Name: 128.210.155.220
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 04/25/2005
Time: 11:40 AM
I used chemicals daily in my research.
first: Michael
last: Oldham
dept: Biological Sciences
workrooms: Lilly B117
email: mloldham@purdue.edu
staffclass: Postdoctoral
jobtitle: postdoc
supervisor: Jue Chen
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: 0.1 M Hydrogen chloride
chem8:
chem2: 10 M Sodium hydroxide
chem9:
chem3: Ethanol
chem10:
chem4: Methanol
chem11:
chem5: Acetic acid
chem12:
chem6: 2-mercapto ethanol
chem13:
chem7: Formaldehyde
chem14:
Remote Name: 128.210.155.171
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1)
Date: 04/25/2005
Time: 11:40 AM
I use these chemicals to grow bacteria, isolate protein, and make crystals of them.
first: James
last: Westbrooks
dept: Biology
workrooms: Lilly B-117
email: jmw@bilbo.bio.purdue.edu
staffclass: Grad student
jobtitle: Research Assistant
supervisor: Jue Chen
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: 0.1 M Hydrogen chloride
chem8: 10 M Sodium hydroxide
chem2: Ethanol
chem9: Methanol
chem3: Acetic acid
chem10: 2-mercapto ethanol
chem4: Formaldehyde
chem11: 30 % Acrylamide/Bis
chem5: N,N,N',N'-Tetramethylethylenediamine
chem12: Ethidium Bromide
chem6: ATP-gamma-S
chem13:
chem7:
chem14:
Remote Name: 128.210.155.206
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.7) Gecko/20050414 Firefox/1.0.3
Date: 04/25/2005
Time: 11:41 AM
These chemicals are used in processes involved in bacterial growth, protein expression, purification and isolation, gel electrophoresis, and nucleotide binding studies.
first: I-Mei
last: Yu
dept: Biological sciences
workrooms: Lilly B117
email: iyu@purdue.edu
staffclass: Grad student
jobtitle: student
supervisor: Jue Chen
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Hydrogen chloride
chem8: methonal
chem2: Ethanol
chem9: 30% Bis/Acrylamide
chem3: Acetic acid
chem10: Sodium hydroxide
chem4: Polyethylene glycol
chem11:
chem5:
chem12:
chem6:
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chem7:
chem14:
Remote Name: 128.210.155.208
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 04/26/2005
Time: 03:34 PM
Expression, purification, and crystallization of proteins. Most used buffers such as Tris-HCl and sodium chloride in the purification procedures. Sizeing columns, His-tag columns, and electrophoresis are used to purify and examine the protein. Crystal trials are mostly set up with commercial screens.
first: Adam
last: Zabell
dept: Biology
workrooms: Lilly Hall, B-201,204,408,409
email: zabell@purdue.edu
staffclass: Postdoctoral
jobtitle: post doctoral research associate
supervisor: Stauffacher
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Ethanol
chem8: Sodium hydroxide
chem2: Hydrochloric acid
chem9: Acrylamide
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
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Remote Name: 128.210.18.221
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X; en) AppleWebKit/312.1 (KHTML, like Gecko) Safari/312
Date: 04/26/2005
Time: 03:45 PM
Mixing simple buffer solutions for use in protein preparation and purification. Use of a French Press to lyse cells, and then standard column chromatography to purify. Once finished, either enzyme kinetics with a UV/Spec or structure determination with X-ray crystallography.
first: Jianbo
last: Diao
dept: Biological Science
workrooms: B117 Lilly Hall
email: jdiao@purdue.edu
staffclass: Postdoctoral
jobtitle: post doctoral research associate
supervisor: Jue Chen
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: ethanol
chem8: Formaldehyde
chem2: 10mg/ml EB
chem9: 2-mercaptoethanol
chem3: 1M acetic acid
chem10: 0.02%sodium Azide
chem4: 10M Potassium hydroxide
chem11: 30 % Acrylamide/Bis
chem5: 5M Sodium acetate
chem12: Hydrogen chlroride
chem6: acetone
chem13: liquid nitrogen
chem7: 1M DTT
chem14: methonal
Remote Name: 128.210.89.230
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 04/26/2005
Time: 04:29 PM
I am working on protein expression. So I using a lot of medium for cell culture and protein purification. I also need identify the expressed protein by biochemistry method which handle a lot of chemical agents.
first: Jue
last: Chen
dept: Biological Sciences
workrooms: B117,B118 (Lilly Hall)
email: chenjue@purdue.edu
staffclass: Faculty
jobtitle: Assistant Professor
supervisor: Jue Chen
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: supervisor
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
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chem6:
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Remote Name: 128.210.48.67
Remote User:
HTTP User Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.6) Gecko/20040413 Debian/1.6-5
Date: 04/27/2005
Time: 10:13 AM
none
first: Alanna
last: Steffen
dept: Biology
workrooms: Lilly B117
email: amsteffe@purdue.edu
staffclass: Grad student
jobtitle: Graduate Student
supervisor: Jue Chen
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: 0.1 M Hydrogen chloride
chem8:
chem2: 10 M Sodium hydroxide
chem9:
chem3: Ethanol
chem10:
chem4: Methanol
chem11:
chem5: Acetic acid
chem12:
chem6: 2-mercapto ethanol
chem13:
chem7: 30 % Acrylamide/Bis
chem14:
Remote Name: 128.210.155.187
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 5.23; Mac_PowerPC)
Date: 04/27/2005
Time: 10:57 AM
I use chemicals for my protein preps and in cloning work.
first: Mahdi
last: Abu-Omar
dept: Chemistry
workrooms: 5170 Brown
email: mabuomar@purdue.edu
staffclass: Faculty
jobtitle: Associate Professor
supervisor: Abu-Omar
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: methylene chloride
chem8: sulfuric acid
chem2: acetonitrile
chem9: organosilanes
chem3: benzene
chem10: hydrogen
chem4: toluene
chem11: nitric acid
chem5: tetrahydrofuran
chem12: organic sulfides
chem6: triflic acid
chem13: chloroform
chem7: perchlorate
chem14: pyridine
Remote Name: 128.210.142.42
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 5.22; Mac_PowerPC)
Date: 04/27/2005
Time: 04:54 PM
Some synthetic work, bench top, vacuum lines, and glovebox. Kinetic experiments on UV-vis, stopped-flow analyzer. Gas evolution kinetics.
first: shiva
last: bhowmik
dept: BIOL
workrooms: B-206 B-209 LILLY
email: sbhowmik@purdue.edu
staffclass: Grad student
jobtitle: graduate student
supervisor: Dr. Jeff T. Bolin
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: ethanol
chem8: methanol
chem2: propanol
chem9: isopropanol
chem3: acetone
chem10: hydrogen
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.48.236
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; FunWebProducts)
Date: 04/27/2005
Time: 07:52 PM
We use chemicals to set up crystallization for biological macromolecule. Hydrogen gas is used to regenerate glove box catalyst.
first: sateesh
last: madhi
dept: chemistry
workrooms: BRWN5125
email: smadhi1@purdue.edu
staffclass: Postdoctoral
jobtitle: post doctoral research associate
supervisor: P. V. Ramachnadran
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.164.130
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.0)
Date: 04/28/2005
Time: 12:24 PM
full time research scholar
first: Bingbing Wu
last: Wu
dept: Biological Sciences
workrooms: Lilly 2-240
email: wub@purdue.edu
staffclass: Grad student
jobtitle: Graduate Student
supervisor: Daniel Suter
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: chlorophorm
chem8: 6N HCl
chem2: ethidium bromide
chem9: isopropanol
chem3: formadehyde
chem10: sodium dodecyl sulfate (SDS)
chem4: sodium hydroxide
chem11: 3-N (Morphollino) Propane sulfonic Acid (MOPS)
chem5: acetic acid
chem12: dimethyl sulfoxide (DMSO)
chem6: diethyl pyrocarbonate (DEPC)
chem13: sodium fluoride
chem7: 40% acrylamide
chem14: L-leucine
Remote Name: 128.210.155.246
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 04/28/2005
Time: 02:40 PM
I am working in a cell biology lab. Most of the time I am dealing with cell cultures and media which contains salts and nutrients. The hazadous or irritant chemicals are usually for molecular biology purpose, such as DNA preparation, and protein study.
first: Elon
last: Ison
dept: Chemistry
workrooms: Brown 5174
email: eaison@purdue.edu
staffclass: Postdoctoral
jobtitle: Post Doc
supervisor: Abu-Omar
CompletedAndUnderstoodTrainingModule: not answered
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Methylene Chloride
chem8:
chem2: Hydrochloric Acid (conc)
chem9:
chem3: Diethyl Ether
chem10:
chem4:
chem11:
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chem12:
chem6:
chem13:
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Remote Name: 128.210.164.164
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.0.3705; .NET CLR 1.1.4322)
Date: 04/28/2005
Time: 03:19 PM
Fulfilling
first: Sara
last: Ess
dept: Biology
workrooms: Lilly B411, B411A, B232B,C
email: sydnizzy@purdue.edu
staffclass: Technical
jobtitle: Lab Assistant IV
supervisor: Barry Wanner
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Ethidium Bromide
chem8: ethanol
chem2: hydrochloric acid
chem9: isopropanol
chem3: sulfuric acid
chem10: methanol
chem4: acrylamide
chem11: phenol:chloroform:isoamyl alcohol
chem5: hydrofluoric acid
chem12: acetic acid
chem6:
chem13:
chem7:
chem14:
Remote Name: 128.210.18.170
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1)
Date: 04/28/2005
Time: 03:21 PM
I mix media and solutions for general use by the researchers in the lab. This involves working with some chemicals listed above (ethanol, methanol, isopropanol, hydrochloric acid, ethidium bromide), using autoclaves, pH meters, centrifuges.
first: Paul
last: Chrisman
dept: Chemistry
workrooms: Brown B151, B178
email: chrismap@purdue.edu
staffclass: Grad student
jobtitle: Graduate assistant
supervisor: Scott Mcluckey
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Methanol
chem8:
chem2: Glacial acetic acid
chem9:
chem3: Ammonium hydroxide
chem10:
chem4: Sodium hydroxide
chem11:
chem5: Acetonitrile
chem12:
chem6: Acetone
chem13:
chem7:
chem14:
Remote Name: 71.98.66.125
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 04/28/2005
Time: 08:50 PM
Most work with chemicals consists of making solutions for use on a mass spectrometer (typically around 10-100 micromolar peptide/protein in a 50/50/1 water/methanol/acetic acid solution, sometimes with a base of some sort instead of acid), usually making at most 1mL of solution. In the lab I work on and assist in the maintenance of several different mass spectrometers, mainly a modified Hitachi 3DQ ion trap, and two modified Finnigan ITMS instruments, as well as a Sciex QSTAR. This also invovles the use and maintanence of a variety of mechanical rough pumps.
first: Militza
last: Carrero-Colon
dept: Biology
workrooms: 4
email: carreroc@purdue.edu
staffclass: Technical
jobtitle: Laboratory Technicia
supervisor: Allan Konopka
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Lead Nitrate
chem8: Chloroform
chem2: Phenol
chem9: Isopropanol
chem3: Ethidium Bromide
chem10: Acrylamide
chem4: Temed
chem11: Hydrochloric Acid
chem5: Acetic Acid
chem12: Surlfuric Acid
chem6: Perchloric Acid
chem13: Sodium Hydroxide
chem7: Nitric Acid
chem14: Urea
Remote Name: 128.210.65.181
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.0.3705)
Date: 04/29/2005
Time: 08:46 AM
As a lab technician I performed different task in the laboratory. The nature of our work as an environmental lab, includes the use of many solutions, made from some of the chemicals listed above. Specifically I work with an environmental bacteria isolated from a lead contanated waste site. I performed growth experimetns at different pH as well as molecular biology analysis.
first: jyothi
last: karangula
dept: chemistry
workrooms: BRWN 5135
email: jyothi@purdue.edu
staffclass: Postdoctoral
jobtitle: post doctoral research associate
supervisor: P.V. Ramachandran
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
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Remote Name: 128.210.164.140
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 04/29/2005
Time: 10:45 AM
I am a full time researcher
first: Peter
last: Kourtev
dept: Biological Sciences
workrooms: Llilly - B229, G127
email: pkourtev@purdue.edu
staffclass: Postdoctoral
jobtitle: postdoctoral associate
supervisor: Allan Konopka
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Potassium chromate
chem8: Choloform
chem2: Phenol
chem9: Xylene
chem3: Isopropanol
chem10: Acyliamide
chem4: TEMED
chem11: Sulfuric acid
chem5: Hydrochloric acid
chem12: Sodium hydroxide
chem6: Acetic acid
chem13: Nitric acid
chem7: Ethydium bromide
chem14: formamide
Remote Name: 128.210.18.183
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax)
Date: 04/29/2005
Time: 11:13 AM
I work in the area of environmental microbiology. The above chemicals are used to make media, to adjust pH of media, and in molecular biology work, for example ethydium bormide is used to staind DNA in agarose gels.
first: Yang
last: Yu
dept: Biological Science
workrooms: Lilly B411
email: yuyang@purdue.edu
staffclass: Grad student
jobtitle: RA
supervisor: Barry L. Wanner
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: ethanol
chem8: isopropanol
chem2: methanol
chem9: formaldehyde
chem3: acetic acid
chem10: sulfuric acid
chem4: ethidium bromide
chem11: hydrochoric acid
chem5: acrylamide
chem12: hydrofluoric acid
chem6: phenol:chloroform:isoamyl alcohol
chem13:
chem7:
chem14:
Remote Name: 128.210.18.27
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1)
Date: 04/29/2005
Time: 01:16 PM
I made some media and solutions for my research work in lab using the above chemicals, which involves in centrifuge, hood, weight balance, pH meter, autoclave and oven.
first: Tai-Wei Joy
last: Tseng
dept: Biological Science
workrooms: Lilly B-411
email: ttseng@purdue.edu
staffclass: Grad student
jobtitle: Graduate student
supervisor: Barry L. Wanner
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: Ethidium Bromide
chem8: phenol:cloroform:isoamyl alcohol
chem2: Ethanol
chem9: Hydrofluoric acid
chem3: Hydrocloric acid
chem10: Acrylamide
chem4: Isopropanol
chem11:
chem5: Sulfuric acid
chem12:
chem6: Methanol
chem13:
chem7: Acetic acid
chem14:
Remote Name: 128.210.18.17
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1)
Date: 04/29/2005
Time: 04:19 PM
Electrophoresis Extract DNA Clone Culture strains Inculate strains
first: Jie
last: Shao
dept: Biological Sciences
workrooms: Lilly Hall B411
email: jieshao@purdue.edu
staffclass: Grad student
jobtitle: Researching assistant
supervisor: Barry L Wanner
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: methanol
chem8: formaldehyde
chem2: acetic acid
chem9: sulfuric acid
chem3: isopropanol
chem10: chloroform
chem4: ethanol
chem11: ethidium bromide
chem5: acrylamide
chem12: methyl alcohol
chem6: dimethylformide
chem13:
chem7:
chem14:
Remote Name: 128.210.18.23
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1)
Date: 04/29/2005
Time: 05:05 PM
I made some media and solutions for my research in the lab using those above chemicals, which involved in hood, weight balance, pH meter, centrifuge, oven and autoclave.
first: Joanna
last: Becker
dept: Biology
workrooms: 4
email: beckerjm@purdue.edu
staffclass: Grad student
jobtitle: Graduate Research Assistant
supervisor: Allan Konopka
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Nitric Acid
chem8: Hydrogen Peroxide
chem2: Acrylamide
chem9: Ethidium Bromide
chem3: TEMED
chem10: Hydrochloric Acid
chem4: Sodium Hydroxide
chem11: Sulfuric Acid
chem5: Lead Nitrate
chem12: Potassium Chromate
chem6: Urea
chem13: Formamide
chem7: Sodium Chloride
chem14: Sodium Bicarbonate
Remote Name: 128.210.65.210
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 04/30/2005
Time: 05:47 PM
In my research I perform a variety of different tasks. Being an environmental lab, much of the work I do involves using chemicals to digest soil in order to determine the native content of various elements. In addition, I use several of the chemicals above in order to complete various molecular biological assays such as DNA extraction.
first: Scott
last: McLuckey
dept: Chemistry
workrooms: B115A, B178
email: mcluckey@purdue.edu
staffclass: Faculty
jobtitle: professor
supervisor: Tim Zwier
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: supervisor
chem1:
chem8:
chem2:
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chem11:
chem5:
chem12:
chem6:
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Remote Name: 128.210.187.150
Remote User:
HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax)
Date: 04/30/2005
Time: 05:53 PM
Operate mass spectrometer.
first: Jeffrey
last: Bolin
dept: Biological Sciences
workrooms: LILY B-206,B-209
email: jtb@purdue.edu
staffclass: Faculty
jobtitle: Professor
supervisor: Jeffrey T. Bolin
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: supervisor
chem1:
chem8:
chem2:
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Remote Name: 128.210.30.174
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 05/01/2005
Time: 10:27 AM
Occasional lab clean-up.
first: PRAVINDRA
last: KUMAR
dept: Biological Sciences
workrooms: B2-07, B-101, LILY HALL
email: kumar9@purdue.edu
staffclass: Postdoctoral
jobtitle: RESEARCH ASSOCIATE
supervisor: DR. J. T. BOLIN
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: topchemicals
chem1: ETHANOL
chem8: 2,2' DI CHLORO BIPHENYL
chem2: METHANOL
chem9: 2,2',5,5' TETRA CHLORO BIPHENYL
chem3: 2-PROPANOL
chem10: 2,4,4' TRI CHLORO BIPHENYL
chem4: POLY ETHYLENE GLYCOLS
chem11: 2,6 DI CHLORO BIPHENYL
chem5: T-BUTANOL
chem12: Pentaerythritol propoxylate
chem6: BIPHENYL
chem13: Pentaerythritol ethoxylate
chem7: ETHYLENE GLYCOL
chem14: 2-METHYL-2,4-PENTANEDIOL
Remote Name: 128.210.48.28
Remote User:
HTTP User Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.6) Gecko/20040413 Debian/1.6-5
Date: 05/01/2005
Time: 12:38 PM
PROTEIN CRYSTALLIZATION AND THREE DIMENSIONAL STRUCTURE DETERMINATION (STRUCTURAL BIOLOGIST)
first: Mustafa
last: Koksal
dept: Biological Sciences
workrooms: LILLY B209
email: mkoksal@purdue.edu
staffclass: Grad student
jobtitle: Student
supervisor: Jeff Bolin
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
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chem6:
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Remote Name: 128.210.48.236
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; FunWebProducts)
Date: 05/01/2005
Time: 02:06 PM
I use chemicals to make solutions to use in crystallization experiments. No chemical reaction is performed.
first: Louis
last: Sherman
dept: Biological Sciences
workrooms: Hansen 131 to 135
email: lsherman@purdue.edue
staffclass: Faculty
jobtitle: Professor
supervisor: Louis Sherman
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: will send attachment
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 12.222.94.187
Remote User:
HTTP User Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X Mach-O; en-US; rv:1.4) Gecko/20030624 Netscape/7.1
Date: 05/01/2005
Time: 05:07 PM
Research in lab revolves around an understanding of photosynthesis in a microbe. These cells are mechanically ruptured and proteins, DNA and RNA are isolated and analyzed. Most chemicals consist of salt solutions, buffers and a few organic chemicals.
first: Joshua
last: Robertson
dept: BIO
workrooms: G-203 Lilly
email: robert29@purdue.edu
staffclass: Undergraduate student
jobtitle: Undergrad Researcher
supervisor: Dr. Ignacio Camarillo
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: 4
chem1:
chem8:
chem2:
chem9:
chem3:
chem10:
chem4:
chem11:
chem5:
chem12:
chem6:
chem13:
chem7:
chem14:
Remote Name: 12.222.139.135
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322)
Date: 05/01/2005
Time: 05:16 PM
analyze protein levels in MCF-7 cancer cells. make acrylamide gels.
first: Eric
last: Harness
dept: Biology
workrooms: 3-2239a
email: eharness@purdue.edu
staffclass: Technical
jobtitle: Lab Tech VIII
supervisor: Dr. William Pak
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Ethanol
chem8: Sodium dodecal sulfate
chem2: Phenol
chem9: Chloroform
chem3: Methanol
chem10: Ethium Bromide
chem4: CO2 gas
chem11: N2 gas
chem5: N2 liquid
chem12: formaldhyde
chem6: Silicone grease
chem13: cotton dust
chem7: Ether
chem14: Hydrogen peroxide
Remote Name: 128.210.155.215
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1)
Date: 05/02/2005
Time: 10:12 AM
I only really work with Ethonal CO2 gas and liquid N2. The rest of the chemicals listed above I am just around on a daily basis. Ethanoal is used to clean instruments. CO2 is to make the flies pass out. The N2 liquid is to freeze stocks.
first: Subhangi
last: Ghosh
dept: Biology
workrooms: Lily-B101
email: ghosh1@purdue.edu
staffclass: Grad student
jobtitle: Student
supervisor: Dr. Jeff Bolin
CompletedAndUnderstoodTrainingModule: yes
InformedOfLabStandardContentsAndLocation: yes
InformedOfCHPLocationAndAvailability: yes
InformedOfSafetyInfoLocationAndAvailability: yes
InformedOfExposureSignsAndSymptoms: yes
InformedOfPELs: yes
TrainedAboutMethodsAndObservationsToDetectRelease: yes
TrainedAboutPhysicalAndHealthHazards: yes
TrainedAboutProtectiveMeasures: yes
TrainedAboutCHPDetails: yes
UnderstandWhomToAskQuestions: yes
ChemicalList: complete
chem1: Ethanol
chem8: Methanol
chem2: 2-Propanol
chem9: 2,4,4' TriChloro Biphenyl
chem3: Poly Ethylene Glycols
chem10: 2,6 DiChloro Biphenyl
chem4: T-Butanol
chem11: Pentaerythritol propoxylate
chem5: 2,2' DiChloro Biphenyl
chem12: Biphenyl
chem6: 2,2' , 5,5' Tetra Chloro Biphenyl
chem13: ethylene glycol
chem7: 2-Methyl-2,4-Pentanediol
chem14: Pentaerythritol ethoxylate
Remote Name: 128.210.48.246
Remote User:
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1)
Date: 05/02/2005
Time: 12:31 PM
I crystallize proteins using some of the reagents described above, freeze them in Liq. N2 and then diffract it by X rays in an Xray instrument.