BME 553     Introduction to Biomedical Optics

Number of credit hours: 3

When offered: Fall semester – every year

Instructor: Ji-Xin Cheng

Prerequisites: Background in basic optics, chemistry, and biology

Topics: Optical Microscopy and Applications to Biomedical Research

Textbook:  Introduction to Biophotonics (John Wiley & Sons, Inc. 2003)

Syllabus

Class 1             Basics of light

Class 2             Light matter interactions

Class 3             Principle of lasers and current laser technology

Class 4             Interactions of cells and tissues with light

Class 5             Principles of optical microscopy

Class 6             Confocal microscopy

Class 7             Labor Day

Class 8             Diagnostic detection with optical spectroscopy and imaging

Class 9             Exam 1

Class 10           Total internal reflection fluorescence microscopy

Class 11           Fluorescence energy transfer and lifetime imaging

Class 12           Literature review

Class 13           Two-photon fluorescence microscopy

Class 14           Nonlinear optics and second and third harmonic generation imaging

Class 15           Literature review

Class 16           Coherent anti-Stokes Raman scattering microscopy-1

Class 17           Coherent anti-Stokes Raman scattering microscopy-2

Class 18           Literature review

Class 19           Exam 2

Class 20           Single molecule fluorescence detection and spectroscopy

Class 21           High-resolution imaging by near field optical microscopy

Class 22           Literature review

Class 23           FTIR spectroscopy and imaging

Class 24           Spontaneous Raman microscopy and applications to diagnostic detection

Class 25           Literature review

Class 26           Light fluctuation correlation spectroscopy

Class 27           Fluorescence recovery after photo bleaching and single particle tracking

Class 27           Literature review        

Class 28           Methods for deep tissue imaging

Class 29           Literature review        

Class 30           Optical and magnetic tweezers

Class 31           Literature review        

Class 32           Light fluctuation correlation spectroscopy

Class 33           Literature review        

Class 34           Biomedical imaging with nano materials

Class 35           Teraherz spectroscopy and imaging   

Class 36           Optical biosensors

Class 37           Photodynamic therapy

Class 38           Exam 3

Class 39           Course review

Class 40           Student presentation of original proposals (final project)

Class 41           Student presentation of original proposals (final project)

Class 42           Student presentation of original proposals (final project)

Class 43           Student presentation of original proposals (final project)

Class 44           Student presentation of original proposals (final project)

Class 45           Final exam

Note for literature review:  For each specific topic, two or three students will present in class a few recently published papers. Each student will submit a short paper that summarizes their comments on the significance, methods and results, strength and weakness of the papers.

Grading:                   Three exams                45%

                                    Literature review         15%

                                    Homework                   20%

                                    Final project                 20%