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Post Doctoral Research Associate (August
2000-March 2002) Application of Ion/Ion
Reactions for “Top Down” Protein Identification and Characterization in a
Quadrupole Ion Trap Mass Spectrometer. Specific Areas of Research: (i)
Examination
of the charge state dependent fragmentation behavior of multiply charged
protein ions. (ii)
Development
of the “ion parking” technique for concentration and purification of multiply
charged precursor ions. (iii)
Application
of ion parking to the gas-phase concentration, purification and
identification of proteins from complex mixtures of E.coli. (iv)
Design,
modification and evaluation of a new quadrupole ion trap mass spectrometer
adapted for ion-ion reaction studies. Publications
arising from Post Doctoral Research: Reid,
G.E. Wells, J.M., Badman, E.R. and McLuckey S.A. (2002) Performance of a
quadrupole ion trap mass spectrometer adapted for ion-ion reaction studies. Int.
J. Mass Spectrom. In Press. He, M,
Reid, G.E. Shang, H-S., Lee, G.U. and McLuckey, S.A. (2002) Product ion
formation from multiple parent ion charge states following gas-phase protein
ion purification and concentration. Anal. Chem. In Press. Engel,
B.J. Pan, P. Reid, G.E., Wells, J.M. and McLuckey, S.A. (2002) Charge state
dependent fragmentation of gaseous protein ions in a quadrupole ion trap:
Bovine ferri-, ferro-, and apo-cytochrome c. Int. J. Mass Spectrom.
In Press. Reid,
G.E. and McLuckey, S.A. (2002) "Top down" protein identification
and characterization via tandem mass spectrometry. In: 'Proteomics: A
Laboratory Manual' by Simpson, R.J. Cold Spring Harbor Laboratory
Press. In Press. Reid,
G.E. and McLuckey, S.A. (2002) “Top down” protein characterization via tandem
mass spectrometry. J. Mass Spectrom. 37: 663–675. Reid,
G.E., Shang, H., Hogan, J., Lee, G.U. and McLuckey S.A. (2002) Gas-phase
concentration, purification and identification of whole proteins from complex
mixtures. J. Am. Chem. Soc. 124: 7353-7362. Stephenson, J.L., McLuckey, S.A., Reid, G.E., Wells,
J.M. and Bundy, J.L. (2002) The detection and identification of intact
proteins from complex mixtures using ion/ion chemistry: Current status and future
implications. Curr. Opin.
Biotechnol. 13: 57-64. Reid,
G.E. and McLuckey, S.A. (2002) Tandem mass spectrometry of ribonuclease A and
B: N-linked glycosylation site analysis of whole protein ions. Anal. Chem.
74: 577-583. McLuckey,
S.A. Reid, G.E. Wells, J.M. (2002) Ion parking during ion/ion reactions in
electrodynamic ion traps. Anal. Chem. 74: 336-346. Newton,
K.A., Chrisman, P.A., Reid, G.E., Wells, J.M. and McLuckey, S.A. (2001)
Gaseous apomyoglobin ion dissociation in a quadrupole ion trap: [M+4H]4+
- [M+21H]21+. Int. J. -.Mass Spectrom. 212: 359-376. Chrisman,
P.A., Newton, K.A., Reid, G.E., Wells, J.M. and McLuckey, S.A. (2001) Loss of
charged versus neutral heme from gaseous holomyoglobin ions. Rapid.
Commun. Mass Spectrom. 15: 2334-2340. Wells,
J.M., Reid, G.E., Engel, B.J., Pan, P. and McLuckey, S.A. (2001) Dissociation
reactions of gaseous ferro-, ferri-, and apo-cytochrome c ions. J. Am. Soc.
Mass Spectrom. 12: 873-876. Reid,
G.E., Wu, J., Chrisman, P.A., Wells, J.M. and McLuckey, S.A. (2001) Charge
state dependance on the sequence analysis of protonated ubiquitin ions by
tandem mass spectrometry and ion/ion reactions. Anal. Chem. 73:
3274-3281. Current
Research Interests: Bioanalytical Mass Spectrometry
and Proteomics. My research focuses on the development and application of novel chemical and mass spectrometry based approaches for the identification, structural characterization and quantitation of proteins present in complex biological mixtures, with particular emphasis on characterization of the proteins and protein complexes involved in signalling pathways associated with cancer development.
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