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Fred E. Regnier
J. H. Law Distinguished Professor—Analytical Chemistry
Email: fregnier@purdue.edu
Phone: 765-494-3878
Office: WTHR 155/BIND 112
The current objective of the laboratory is to develop integrated analytical systems for the analysis and characterization of complex protein mixtures using multidimensional separation systems (1,2) and mass spectrometry. The current initiative is to develop an automated, “one-pass” system that would identify proteins from cellular extracts that are in regulatory flux. A wide variety of stimuli are being examined ranging from cancer to specific diseases. Both conventional and chip based separation systems are being developed for this purpose.
The chips based systems use micromachining to fabricate in situ all the components of multiple multidimensional separation systems on a single quartz wafer (3). This includes millions of micron size collocated monoliths support structures (COMMOS) (see figure below), fluid distributors, mixers, sample inlets, a solvent formation system, chemical reaction vessels, and detector flow cells. Through deep reactive ion etching, channels ranging down to 1.5 x 10 mmm in cross section have been fabricated. Subsequent to sample introduction into the chip, reduction and alkylation, proteolysis, reversed phase chromtography, and transport to a mass spectrometer will all be carried out within channel systems in the chip. The broad objective of the on-going research is to optimize these new miniaturized systems for protein characterization.
Education
B.S., 1960, Nebraska State College; Ph.D., 1965, Oklahoma State University; Postdoctoral, University of Chicago, 1966; Postdoctoral, Harvard University, 1967.Recognitions
- Outstanding Commercialization Award for Purdue University Faculty, 2006
Selected Publications
- Jung K. Y.;Cho, W. R.;Regnier, F. E., Glycoproteomics of Plasma Based on Narrow Selectivity Lectin Affinity Chromatography . Journal of Proteome Research 2009 , 8 , 643-650.
- Yang W. C.;Regnier, F. E.;Adamec, J., Comparative metabolite profiling of carboxylic acids in rat urine by CE-ESI MS/MS through positively pre-charged and H-2-coded derivatization . Electrophoresis 2008 , 29 , 4549-4560.
- Yang W. C.;Sedlak, M.;Regnier, F. E.;Mosier, N.;Ho, N.;Adamec, J., Simultaneous Quantification of Metabolites Involved in Central Carbon and Energy Metabolism Using Reversed-Phase Liquid Chromatography-Mass Spectrometry and in Vitro C-13 Labeling . Analytical Chemistry 2008 , 80 , 9508-9516.
- Wilson C. R.;Regnier, F. E.;Knapp, D. W.;Raskin, R. E.;Andrews, D. A.;Hooser, S. B., Glycoproteomic profiling of serum peptides in canine lymphoma and transitional cell carcinoma . Veterinary and Comparative Oncology 2008 , 6 , 171-181.