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| Varian INOVA 300 with LC-NMR setup shown at the left. |
"Lizzy" is a Varian INOVA 300MHz spectrometer used for a variety of liquids and solids experiments. The console for this machine is shared between two magnets, both wide-bore Oxford cryostats. This spectrometer finds use in both our bioanalytical and solid-state characterization projects. It is interfaced to a Shimadzu LC system with a home-built online pre-concentration setup, as well as a Gilson 215 robotic liquids handler for high-throughput sample movement and analysis. We also use the instrument for testing and characterization of our home-built NMR probes. On the solids side, this system is used for standard CP-MAS or static acquisitions and has been used in a number of in situ photocatalytic experiments. |
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| Oxford 300MHz magnet shared through our INOVA console. |
Our second 7.4T magnet (the "Blue Ox") is used primarily for solids experiments. Static and CP-MAS experiments are run on this instrument along with some custom pulse sequences and inspection of non-traditional nuclei. A high frequency spin controller is also used on this system. |
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| Resonance Instruments 200MHz NMR with imaging capability. |
The RI-200 is a wide-bore solids instrument with triple-axis gradients capable of imaging. This instrument is used in our lower-field 129Xe optical pumping experiments. |
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| Oxford 500MHz magnet with home-built console. |
Our 500MHz magnet was recently acquired from UC Irvine and relocated to our lab. Currently, this narrow-bore magnet is connected to a home-built console with a Tecmag pulse-programmer and CMX/Varian amps and pre-amps and various other components. This instrument finds some use in our instrumentation research efforts, and will be used increasingly for metaboloite studies in the future. |
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| Shimadzu LC interfaced to our 300MHz NMR. |
Our LC-NMR system cosnsists of a standard Shimadzu LC system in-line with a microcoil probe in our 300MHz INOVA instrument. Between the LC and probe is a custom LabVIEW-controlled valving setup for swicthing and storing chromatographic eluates and moving them to the probe detection cell. This system is used extensivley in our research involving signal enhancement by sample preconcentration. |
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| Bruker Avance III 800MHz NMR located in the Purdue NMR Facility |
The Bruker Avance III 800MHz NMR spectrometer is a new addition to the Purdue Interdepartmental NMR Facility. It will extend the sensitivity and resolution of experiments for a number of projects related to our ongoing Metabalomics research. |
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| Bruker Avance 500MHz Spectrometer with cryoprobe. |
The DRX 500-1 is a Bruker Avance spectrometer located in the Purdue Interdepartmental NMR Facility. This spectrometer is equipped with a 5mm cryoprobe and exhibits 1H sensitivity of greater than 3000:1. This instrument is used heavily for routine NMR in our metabolomics projects. |
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| Bruker DRX 500-2 spectrometer located in the Heine Pharmacy Bldg. |
Another heavily used PINMRF instrument is the DRX 500-2. This spectrometer is identical to the DRX 500-1 except that it does not have a cryoprobe. This instrument is equipped with TXI and BBO probes that provide excellent sensitivity for our multidimensional NMR experiments. It also provides excellent broadband tunability for running various non-traditional nuclei. |
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| CMX 400 spectrometer for solids experiments. |
The CMX-400 is a wide bore instrument for NMR of solids located in the Purdue NMR Facility. It is equipped with a variety of static and MAS probes, from 2 mm to 5 mm . High rate spinning and broadband tunability are features of this setup. Currently the instrument is used extensively in our 129Xe optical pumping research, as well as metabolite studies on whole tissue samples and extracts. |
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| Inert environment glove box in our synthesis lab. |
Our group's synthesis lab is where the majority of wet chemistry takes place. It consists of bench and hood space along with an inert environment glove box and a vacuum rack system. |
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| Finnigan ESI-MS for use in metabolomics studies. |
The Finnigan MS is an quadrapole ion trap instrument with an electrospray source. It finds use in the mass analysis of metabolites in biological fluids. It used in the investigation of metabolic perturbations through correlation with complimentary NMR data. |
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| The probe room is where electronic work is done. |
The Probe Room is comprised of bench space for electronic work, a computer for circuit modeling and space for cosntruction of probes and wet chemistry preparation. |
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