Location

The Lab is located in Room 3125 of the Brown Chemistry Building.

Hours

Monday - Friday 9:30am - 5:30pm

Staff

Nancy L. Petretic, B.S. Biology
Laboratory Technician
petretin@purdue.edu
765-494-5259
BRWN 3125

Using Facility

• Active Cell Culture Lab Membership Required*
• CHP and PPE Training Required
• Online BSL-2 and Handling Human Cell Lines (through REM web site) included in training
• Contact the manager directly to schedule training
• Upon completion of training, students may use instruments independently for their experiments.

* Faculty should arrange method of payment through the manager and Chemistry business office.

Membership Rates

• 12 month
  - Chemistry affiliation: $4000
  - External affiliation: $5000
• 6 month
  - Chemistry affiliation: $2000
  - External affiliation: $2500
• Cell culture training only
  - Chemistry affiliation: $500
  - External affiliation: $600
• Autoclave training only
  - Chemistry affiliation: no charge

More Information

Centrifuge Safety

Equipment

• Flow cytometer, FACS Calibur, Becton Dickinson with up to 4 color laser detection capability, two Apple Power Mac G4 computers with studio display, Cell Quest software, file-sharing activated, HP Business Inkjet 2280tn color printer
• Five Biological Safety Cabinets Class II A, Baker Co. and Contamination Control, Inc.
• Five Cancer Cell Culture Only Incubators (Carbon dioxide, 37 degree), Forma Scientific and Queue
• Microscope, Inverted, Olympus CK-40
• Automated Coulter Particle Counter, Beckman Z1
• Centrifuge, tabletop, low speed, Sorvall RT 6000B
• Liquid Scintillation Analyzer, TriCarb 1600 CA, Packard with computer and printer

Recent Publications using lab data

• Wei, Q.; Song, H.-M.; Leonov, A. P.; Hale, J.; Oh, D.; Ong, Q. K.; Ritchie, K.; Wei, A. “Gyromagnetic Imaging: Dynamic Optical Contrast Using Gold Nanostars with Magnetic Cores.” (submitted 2/17/2009)
• Leonov, A. P.; Zheng, J.; Clogston, J. D.; Stern, S. T.; Patri, A. K.; Wei, A. “Detoxification of Gold Nanorods by Treatment With Polystyrenesulfonate.” ACS Nano 2008, 2, 2481-88.
• SeungHyeon Ko, Haipeng Liu, Yi Chen and Chengde Mao, "DNA Nanotubes as Cominatorial Vehicles for Cellular Delivery," Biomacromolecules, 2008, 9 (11), pp 3039-3043.
• Lim HW, Lee J, Hillsamer P, Kim CH. "Human Th17 cells share major trafficking receptors with both polarized effector T cells and FOXP3+ regulatory T cells," J Immunol, 2008 Jan 1;180(1):122-9.
• Lim HW, Kim CH. "Loss of IL-7 Receptor alpha on CD4+ T Cells Defines Terminally Differentiated B Cell-Helping Effector T Cells in a B Cell-Rich Lymphoid Tissue." J Immunol. 2007, 179:7448-56.
• Sun KH, de Pablo Y, Vincent F, Johnson EO, Chavers AK, Shah K. (2008) Novel Genetic Tools Reveal Cdk5's Major Role in Golgi Fragmentation in Alzheimer's Disease. Mol Biol Cell. 19(7):3052-69.