Biomolecular NMR

CHM 615: Fundamentals of modern NMR spectroscopy

Nikolai Skrynnikov

• Spin magnetization. Vector model. Rotating frame of reference. Pulses.
• Fourier transformation. Spectral processing.
• Two-dimensional spectroscopy. Example: EXSY.
• Rotations. Tilted frame. Off-resonance irradiation.
• Amplitude- and phase-modulated pulses. Decoupling.
• Spin density matrix. Master equation for spin density matrix. Product operators.
• Analysis of pulse sequences. Examples: HSQC, COSY.
• Redfield relaxation theory. Correlation functions. Examples: T1, NOESY.
• Use of NMR for protein structure elucidation.

CHM 631: Biomolecular NMR spectroscopy

Nikolai Skrynnikov

• Overview of fundamentals. Vector model. Spin density matrix. Product operators.
• Analysis of sequences. Examples: HSQC, CT-HSQC, enhanced sensitivity HSQC.
• Spectral assignment experiments. Examples: HNCO, CBCANH.
• Overhauser effect. Example: 13C/15N-resolved NOESY.
• Software and protocols for protein structure calculation.
• Residual dipolar coupling measurements. Example: IPAP-HSQC.
• Principles of TROSY spectroscopy. Example: IPAP-HSQC.
• Relaxation measurements. Models of motion. Example: 15N T1, T1_ρ, NOE.

CHM 616/MCMP 616: Advanced experimental NMR spectroscopy

John Harwood

Please, contact NMR facility (dbertram@purdue.edu) for information about this summer course and hands-on training sessions.