Nikolai Skrynnikov
- Spin magnetization. Vector model. Rotating frame of reference. Pulses.
- Fourier transformation. Spectral processing.
- Two-dimensional spectroscopy. Example: EXSY.
- Rotations. Tilted frame. Off-resonance irradiation.
- Amplitude- and phase-modulated pulses. Decoupling.
- Spin density matrix. Master equation for spin density matrix. Product operators.
- Analysis of pulse sequences. Examples: HSQC, COSY.
- Redfield relaxation theory. Correlation functions. Examples: T1, NOESY.
- Use of NMR for protein structure elucidation.
Nikolai Skrynnikov
- Overview of fundamentals. Vector model. Spin density matrix. Product operators.
- Analysis of sequences. Examples: HSQC, CT-HSQC, enhanced sensitivity HSQC.
- Spectral assignment experiments. Examples: HNCO, CBCANH.
- Overhauser effect. Example: 13C/15N-resolved NOESY.
- Software and protocols for protein structure calculation.
- Residual dipolar coupling measurements. Example: IPAP-HSQC.
- Principles of TROSY spectroscopy. Example: IPAP-HSQC.
- Relaxation measurements. Models of motion. Example: 15N T1, T1ρ, NOE.
John Harwood
Please, contact NMR facility (dbertram@purdue.edu) for information about this summer course and hands-on training sessions.
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