Fang Yan

Ph.D., Analytical Chemistry, University of Michigan, Ann Arbor, 05/2003 (Advisor: Professor David M Lubman)

    Thesis Title: The Developments of Proteosep Technique and Protein Microarrays as well as Their Applications to Cancer Proteomics with Mass Spectrometric Method (Proteosep technique is the winner of R&D 100 award in 2002)

M.S.,  Analytical Chemistry, Fudan University, Shanghai, P.R.China, 07/1998 (Advisor: Professor Jiaqi Deng) 

B.S.,   Chemistry/Computer Science (Minor), East China Normal U., Shanghai, P.R.China, 07/1995

Research Experience:

Purdue Univeristy (2003 - 2004):

   "Top-down" proteomic study of Saccharomyces Cerevisiae:

   Developing LC-based separation and purification methods;

    Appling Ion/Ion reactions to intact proteins- identification and characterization using quadruple ion trap mass spectrometers.

University of Michigan (1999 -2003):

General research: Developed new proteomic methodologies and realized their applications to cancer proteomics.

Part 1. Developed novel multidimensional LC-based chromatofocusing-NPS-RP-HPLC separation method; Protein microarray for screening cellular and tissue proteins in a high throughput and highly parallel fashion.

Part 2. Applications of novel LC-based separation methods to cancer research:

    - Human Breast Cancer Project: The study of the change in proteomic patterns of  human breast cancer cells at  different stages of cancer  progression                                                

    - Human Colon Cancer Project: The study of drug effects as a function of concentration vs. time on the HCT116 cells

    - Human Prostate Cancer project:  The search for multiple tumor-specific protein biomarkers in human sera via protein microarray platform.

Selected Publications and Patents Arising from Ph.D Research:

"Protein Microarrays Using Liquid Phase Fractionation of Cell Lysates",

Yan F, Screekumar A, Laxman B, Chinnaiyan AM, Lubman DM, Proteomics, 2003, 3(7), 1228-1235.

"A Comparison of Drug Treated and Untreated Colon Cancer Cells Using a 2-D Liquid Separations Mapping Method Based Upon Chromatofocusing pI Fractionation", 

Yan F, Subramanian B, Nakeff A, Parus S, Lubman DM, Barder TJ, Anal. Chem., 2003, 75(10), 2299-2308.

"Proteomics Using 2-D liquid separations of Intact Proteins From Whole Cell Lysates",

Zhu K, Yan F., O'Neil KA., Lin L. Barder TJ., Lubman DM., Current protocols in protein science,

Accepted for publication in Current Protocols in Protein Science, 2004 book series.

"Chromatofocusing-nonporous-RP-HPLC-ESI-oaTOF MS: A Novel 3-D Protein Analysis System",

US Patent Application No. 09/778,548, Yan F., Chong BE, Barder TJ, Lubman DM, 2002

"2-D liquid separations/Mass Mapping of Proteins From Human Cancer Cell lysates"A review paper,

Lubman DM, Kachman M, Wang HX, Gong SY, Yan F, Hamler R, O'Neil K, Zhu K, Buchanan N, the Journal of Chromatography B, 782(1-2), 2002, 183-196

"Chromatofocusing nonporous reversed-phase high-performance liquid chromatography electrospray ionization time-of-flight mass spectrometry of proteins from human breast cancer whole cell lysates: a novel two-dimensional liquid chromatography/mass spectrometry method", Chong BE, Yan F, Lubman DM, Miller FR, Rapid Commun. Mass Spectrom., 15(4), 2001, 291-296.

Presentations:

"Protein Microarray for Prostate Cancer Using Liquid Proteome Fractionation",

ASMS oral presentation, June, 2003, Montreal, Canada

"The Application of Chromatofocusing-Multi-Dimensional-Liquid-Phase Separation Method in the Study of Drug-Treated Colon Cancer HCT116 Cells", ASMS poster, June, 2002, Orlando, FL

"A Two Dimensional Protein Mapping: Chromatofocusing-Nonporous-Reverse-Phase-HPLC ESI-TOF Mass Spectrometry",

ASMS poster, June, 2001, Chicago, IL

Affiliations: