Lyon Lab

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Structure and regulation of phospholipase Cβ and ε at the membrane. Muralidharan, K., Van Camp, M.M., and Lyon, A.M.* Chem. Phys. Lipids 235 (2021). PMID: 33422547.

Q's the Boss: Gαq Regulations Gi-Mediated Calcium Release through PLCb. Fisher, I.J. and Lyon, A.M.* Mol. Cell. 80(6), 933-934 (2020). PMID: 33338407.

Functional and strutural characterization of allosteric activation of phospholipase Cε by Rap1A. Sieng, M., Selvia, A.F., Garland-Kuntz, E.E., Hopkins, J.B., Fisher, I.J., Marti, A.T., and Lyon, A.M.* J. Biol. Chem. 295(49), 16562-16571 (2020). PMID: 32948655.

Structure of phospholipase Cε reveals an integrated RA1 domain and previously unidentified regulatory elements. Rugema, N.Y., Garland-Kuntz, E.E., Sieng, M., Muralidharan, K., Van Camp, M.M., O'Neill, H., Mbongo, W., Selvia, A.F., Marti, A.T., Everly, A., McKenzie, E., and Lyon, A.M. Commun. Biol. 3(1), 445 (2020). PMID: 32796910.

q and the Phosphlipase Cβ3 X–Y Linker Regulate Adsoprtion and Activity on Compressed Lipid Monolayers. Hudson, B.N, Jessup, R.E, Prahalad, K.K., and Lyon, A.M.* Biochemistry 58(32), 3454-3467 (2019). PMID:31322863.

Intramolecular electrostatic interactions contribute to phospholipase Cβ3 autoinhibition. Esquina, C.M., Garland-Kuntz, E.E., Goldfarb, D., McDonald, E.K., Hudson, B.N., Lyon, A.M.* Cell. Signal. 62 (2019). PMID: 31254604.

Quantifying acute fueld and respiration dependent pH homeostasis in live cells using the mCherryTYG mutant as a fluorescence lifetime sensor. Haynes, E.P., Rajendran, M., Henning, C.K., Mishra, A., Lyon, A.M., Tantama, M.* Anal. Chem. 91(13), 8466-8475 (2019). PMID: 31247720.

High-resolution structure of RGS17 suggests a role for Ca2+ in promoting the GTPase-activating protein activity by RZ subfamily members. Sieng, M., Hayes, M.P., O'Brien, J.B., Fowler, C.A., Houtman, J.C., Roman, D.L., and Lyon, A.M.* J. Biol. Chem. 294(20), 8148-8160 (2019). PMID: 30940727.

Direct obversation of conformational dyanmics of the PH domain in phospholipases Cε and β may contribute to subfamily-specific roles in regulation. Garland-Kuntz, E.E., Vago, F.S., Sieng, M., Van Camp, M., Chakravarthy, S., Blaine, A., Corpstein, C., Jiang, W., Lyon, A.M.*  J. Biol. Chem. 293(45), 17477-17490 (2018). PMID: 30242131.

G protein βγ subunits direct interact with and activation phospholipase Cε. Madukwe, J.C., Garland-Kuntz, E.E., Lyon, A.M., Smrcka, A.V.* J. Biol. Chem. 293(17), 6387-6397 (2018). PMID: 29535186.

Defect in phosphoinositide signalling through a homozygous variant in PLCB3 causes a new form of spondylometaphyseal dysplasia with corneal dystrophy. Ben-Salem, S., Robbins, S.M., Sobreira, L.M., Lyon, A.M., Al-Shamsi, A.M., Islam, B.K., John, A., Thachillath, P., Al Hamed, S, Valle, D., Ali, B.R., and Al-Gazali, L.* J. Med. Genet. 55(2), 122-130 (2017). PMID: 29122926.

Phospholipase Cβ3 membrane adsorption and activation is regulated by its C-terminal domains and PIP2. Hudson, B.N., Hyun, S.-H., Thompson, D.T., and Lyon, A.M.* Biochemistry 56(41), 5604-5614 (2017). PMID: 28945350

Molecular Mechanisms of Phospholipase C β3 Autoinhibition. Lyon, A.M., Begley, J.A., Manett, T.M., and Tesmer, J.J.G.* Structure 22, 1844-1854 (2014). PMID:25435326

Strike a Pose: Gαq Complexes at the Membrane. Lyon, A.M., Taylor, V.G., and Tesmer, J.J.G.* Trends Pharmacol. Sci. 35(1), 23-30 (2014). PMID:24287282

Structural Insights into Phospholipase C-β Function. Lyon, A.M.* and Tesmer, J.J.G. Mol. Pharm. 84(4), 488-500 (2013). PMID:23880553

Full-length Gαq-phospholipase C-β3 structure reveals interfaces of the C-terminal coiled-coil domain. Lyon, A.M., Dutta, S., Boguth, C.A., Skiniotis, G., and Tesmer, J.J.G.* Nat. Struct. Mol. Biol. 20(3), 355-362 (2013). PMID:23377541

An autoinhibitory helix in the C-terminal region of phospholipase C-β mediates Gαq activation. Lyon, A.M., Tesmer, V.M., Dhamsania, V.D., Thal, D.M., Gutierrez, J., Chowdhury, K.C., Northup, J.K., and Tesmer, J.J.G.* Nat. Struct. Mol. Biol. 18(9), 999-1005 (2011). PMID: 21822282

Bruno protein contains an expanded RNA recognition motif. Lyon, A.M., Reveal, B.S., Macdonald, P.M., and Hoffman, D.W.* Biochemistry 48(51), 12202-12212 (2009). PMID: 19919093

The structure of a eukaryotic translation initiation factor-4E from wheat reveals a novel disulfide bond. Monzingo, A.F., Dhaliwal, S., Dutt-Chaudhuri, A., Lyon, A., Sadow, J.H., Hoffman, D.W., Robertus, J.D., and Browning, K.S.* Plant Physiol. 143(4), 1504-1518 (2007). PMID:17322339