Biomolecular NMR

Purdue University

CHM 615: Fundamentals of modern NMR spectroscopy

Nikolai Skrynnikov

  • Spin magnetization. Vector model. Rotating frame of reference. Pulses.
  • Fourier transformation. Spectral processing.
  • Two-dimensional spectroscopy. Example: EXSY.
  • Rotations. Tilted frame. Off-resonance irradiation.
  • Amplitude- and phase-modulated pulses. Decoupling.
  • Spin density matrix. Master equation for spin density matrix. Product operators.
  • Analysis of pulse sequences. Examples: HSQC, COSY.
  • Redfield relaxation theory. Correlation functions. Examples: T1, NOESY.
  • Use of NMR for protein structure elucidation.

CHM 631: Biomolecular NMR spectroscopy

Nikolai Skrynnikov

  • Overview of fundamentals. Vector model. Spin density matrix. Product operators.
  • Analysis of sequences. Examples: HSQC, CT-HSQC, enhanced sensitivity HSQC.
  • Spectral assignment experiments. Examples: HNCO, CBCANH.
  • Overhauser effect. Example: 13C/15N-resolved NOESY.
  • Software and protocols for protein structure calculation.
  • Residual dipolar coupling measurements. Example: IPAP-HSQC.
  • Principles of TROSY spectroscopy. Example: IPAP-HSQC.
  • Relaxation measurements. Models of motion. Example: 15N T1, T1ρ, NOE.

CHM 616/MCMP 616: Advanced experimental NMR spectroscopy

John Harwood

Please, contact NMR facility ( for information about this summer course and hands-on training sessions.

Find us at WTHR 333A & 352 in WTHR building (map), phone: (765) 496-3324