Molecular Targets For Cancer & Aging Therapeutics: Research Overview
The overall goal of my research program is to understand the mechanisms and roles of important eukaryotic integral membrane proteins that are fundamental to human health and disease. My multidisciplinary work successfully integrates the tools of biochemistry, molecular biology, cell biology and biophysical chemistry to define how these membrane proteins recognize their substrates and how they operate at the molecular level. We also develop ways to use our mechanistic knowledge to create pharmacological agents to modulate the activities of these important proteins. Specifically, I focus on three major areas: 1) the membrane-associated enzymes involved in the posttranslational processing of ) the human ATP binding cassette (ABC) transporters ABCG2 and P-glycoprotein, and (3) drug discovery for inhibitors of human Icmt and for human ABC transporters at the blood-brain barrier.
Namanja, H.A., Emmert, D., Pires, M.M., Hrycyna, C.A.*, and Chmielewski, J.*: Inhibition of Human P-glycoprotein Transport and Substrate Binding Using a Galantamine Dimer, Biochem. Biophys. Res. Comm., 388: 672 - 676, 2009.
Griggs, A.M., Hahne, K., and Hrycyna, C.A.*: Functional Oligomerization of the Saccharomyces cerevisiae Isoprenylcysteine Carboxyl Methyltransferase, Ste14p, J. Biol. Chem., in press, 2010.