Isomer Resolved Imaging via online derivatization of C=C bonds

Online derivatization of C=C bonds enables the imaging of positional lipid isomers that would be otherwise plotted as a mixture using conventional MSI. Our method uses singlet oxygen which selectively reacts with C=C in lipids to form lipid hydroperoxides. The hydroperoxides products yield diagnostic fragments that reveal the position of the C=C in the original lipid. This method is inexpensive and fast. Moreover, it will provide insights in the role of positional isomers in the origin and progression of various diseases.

Publications

Unsihuay D, Su P, Hu H, Qiu J, Kuang S, Li Y, Sun X, Dey SK, Laskin J. Angew. Chem. Int. Ed. 2021; 60(14):7559-7563.